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pEYFP-N1-HsSmg6_1-1238-siRNAres_I

Plasmid

#146558

Purpose

Mammalian Expression of HsSmg6_1-1238-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-1238-siRNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and one non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-HsSmg6-del577-816_I

Plasmid

#146563

Purpose

Mammalian Expression of HsSmg6-del577-816

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-del577-816 (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-HsSmg6-siRNAres_I

Plasmid

#146565

Purpose

Mammalian Expression of HsSmg6-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-siRNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg6_1-576-V5-SBP-MBP_I

Plasmid

#146569

Purpose

Mammalian Expression of HsSmg6_1-576

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-576 (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-ATG13 (1-197)

Plasmid

#188074

Purpose

To make ATG101/ATG13 HORMA complex for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

codon-optimized ATG13 (1-197)

Promoter

CMV

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC

Plasmid

#148402

Purpose

Insect Expression of DmNot1_1674-1962-dsRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1_1674-1962-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

2 silent mutations compared to the sequence given…

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMextli-M605D_AB

Plasmid

#148344

Purpose

Insect Expression of DmMextli-M605D

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli-M605D (mxt Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1711-2505-dsRNAres_R

Plasmid

#147433

Purpose

Insect Expression of DmNot1_1711-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1711-2505-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV495

Plasmid

#177702

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Mutation

Promoter

RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1148_I

Plasmid

#146604

Purpose

Insect Expression of DmNot1_1-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1148 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

4 silent and two non silent N605S, K759M mutation…

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_102-330-V5His6_D

Plasmid

#146129

Purpose

Insect Expression of DmEDC3_102-330

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3_102-330 (Edc3 Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_1-274_AG

Plasmid

#148750

Purpose

Mammalian Expression of HsNot4_1-274

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_1-274 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(T50A)

Plasmid

#112727

Purpose

Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(T50A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

T50A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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PatoM-OG241

Plasmid

#72842

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoM

Use

Tags

Expression

Bacterial

Mutation

Promoter

PatoM

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPn

Plasmid

#87165

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Use

Tags

YFPn

Expression

Mutation

Promoter

35s

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

Plasmid

#193942

Purpose

a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.

Depositor

Greg Delgoffe

Article

Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122

Insert

heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAc-C Med12His

Plasmid

#49240

Purpose

expresses human Med12 with His tag in insect cells

Depositor

Dylan Taatjes

Article

Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

Insert

Med12 (MED12 Human)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (WT)

Plasmid

#42202

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

c-SRC (SRC Human)

Use

Tags

Expression

Mammalian

Mutation

L176F mutation compared to reference sequence NM_…

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_LC_WT

Plasmid

#98653

Purpose

Expresses MBP-tagged FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163

Plasmid

#127192

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 (FUS Human)

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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