Addgene

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pFA6a-HBH-TRP1

Plasmid

#26874

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag

Use

Pcr tagging yeast

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp8a

Plasmid

#163592

Purpose

Expresses BMP-8A in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp8a (Bmp8a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-HTB-TRP1

Plasmid

#26877

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pCXN2-mVIN

Plasmid

#11776

Depositor

Eileen Adamson

Article

Xu et al J Cell Sci. 1998 Jun . 111 ( Pt 11)():15

Insert

vinculin (Vcl Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only
pmMaple2-CAM

Plasmid

#101149

Purpose

Fluorescent protein for superresolution imaging

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

E. coli codon optimized mMaple2

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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Actinin-C-stFRET

Plasmid

#61104

Purpose

Expression of α-actinin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac GST-SAS6

Plasmid

#80273

Purpose

baculovirus creation plasmid for GST-SAS6

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

SAS6 (SASS6 Human)

Use

Tags

GST

Expression

Insect

Mutation

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pSCW02

Plasmid

#72301

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGFP-HBx NESM

Plasmid

#24932

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

L98A,G99A,L100A

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pCS2-SP-FKBP-mCherry-TM-V5

Plasmid

#186226

Purpose

mRNA synthesis of rapalog-induced cell adhesion molecule

Depositor

Yoshihiro Morishita

Article

Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

Insert

SP-FKBP-mCherry-TM-V5

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCYB-TnsA

Plasmid

#17356

Depositor

Nancy Craig

Article

Hickman et al Mol Cell. 2000 Jun . 5(6):1025-34.

Insert

TnsA (tnsA E. coli transposon Tn7)

Use

Tags

Intein-Chitin binding domain

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pRS416-PHO5-GFP-Osh3

Plasmid

#58839

Purpose

Expresses GFP-Tagged Osh3 in yeast

Depositor

Tim Levine, Sean Munro

Article

Levine et al Mol Biol Cell. 2001 Jun;12(6):1633-44.

Insert

Osh3p (OSH3 Budding Yeast)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pNM3749

Plasmid

#154108

Purpose

loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3

Use

Cre/Lox; Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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Omega Destination-CMV::ELuc:bGH

Plasmid

#124528

Purpose

Omega destination vector, that has a CMV:ELUC:bgH in the backbone

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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PH-Gab2-GFP

Plasmid

#35147

Depositor

Sergio Grinstein

Article

Gu et al J Cell Biol. 2003 Jun 23;161(6):1151-61.

Insert

Gab2 PH Domain(x2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains two copies of the PH domain of Gab2: aa …

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only
pGR-ECK120033127

Plasmid

#46011

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120033127 Terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pAGM49981

Plasmid

#169087

Purpose

MoClo level 0 BvADHα coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

BvADHα

Use

Synthetic Biology; Moclo cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG_OriM

Plasmid

#110097

Purpose

Analogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)

Plasmid

#50031

Purpose

Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

PM-SrtA(strep)

Use

Tags

C-HA

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1

Plasmid

#21684

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Decapping protein 1 (Dcp1 Fly)

Use

Drosophila expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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