-
Plasmid#217364PurposeExpresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmidDepositorInsert4 copies of pyrrolysyl-tRNA mutant PyOtR
UseBaculoviralTagsExpressionInsect and MammalianMutationPromoterU6Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA
Plasmid#184636PurposeExpresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGat1 (Slc6a1 Mouse)
UseAAVTagsExpressionMutationGat1 gene with Arginine 69 changed to Lysine, to …PromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+Q111R
Plasmid#167173PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cellsDepositorInsertsATP1A1-S+Q111R
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged Q at 111 to RPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+N122D
Plasmid#167174PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cellsDepositorInsertsATP1A1-S+N122D
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged N at 111 to DPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mWasabi-ADAP1
Plasmid#163906PurposeFull length mouse ADAP1 with N terminal fluorescent tag for purification from insect cellsDepositorInsertADAP1 (Adap1 Mouse)
UseTagsmWasabi fluorescent tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-mTurquoise2-IL-1beta-mNeonGreen
Plasmid#166783PurposeFRET analysis (pro-IL-1beta cleavage) in living cellsDepositorInsertmTurquoise2-IL-1b-mNeongreen (IL1B Human)
UseLentiviralTagsmNeonGreen and mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-mTurquoise2-Caspase-1-mNeonGreen
Plasmid#166784PurposeFRET analysis (pro caspase-1 cleavage) in living cellsDepositorInsertmTurquoise2-caspase-1-mNeonGreen (CASP1 Human)
UseLentiviralTagsmNeonGreen and mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.JARID2
Plasmid#125166PurposeExpresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertJARID2 (JARID2 Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV TIP60 delta HAT
Plasmid#78793PurposeTo overexpress TIP60 delta HAT in Mammalian CellDepositorInsertTIP60 (KAT5 Human)
UseTags3xFLAGExpressionMammalianMutationHAT domain deletion mutationPromoterCMVAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EPOP
Plasmid#125170PurposeExpresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertEPOP (EPOP Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX301-ARF6-T157N-TagRFP
Plasmid#162030PurposeFast cycling ARF mutantDepositorInsertARF6 (ARF6 Human)
UseLentiviralTagsExpressionMutationT151NPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
KAT5 sgRNA1
Plasmid#138187Purpose3rd generation lentiviral gRNA plasmid targeting human KAT5DepositorInsertKAT5 (KAT5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
KAT5 sgRNA2
Plasmid#138188Purpose3rd generation lentiviral gRNA plasmid targeting human KAT5DepositorInsertKAT5 (KAT5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-EphB4-SV40-GFP (B4G)
Plasmid#65444PurposeExpression of human EPHB4 and GFP in mammalian cellsDepositorInsertEPHB4 (EPHB4 Human)
UseLentiviralTagsSV40-EGFPExpressionMammalianMutationPromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
KC1D_HUMAN_D0
Plasmid#79723PurposeThis plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKC1D (CSNK1D Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHB4-2A-EGFP
Plasmid#139968PurposeDonor vector to knockin EGFP (separated by 2A) into EPHB4 alleleDepositorInsertHuman EPHB4 knockin homology arms (EPHB4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
R-Q111R-N122D
Plasmid#167178PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cellsDepositorInsertsATP1A1-R-Q111R-N122D
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged R at 111 to Q and changed D at 122 to NPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV TIP60 365-513
Plasmid#78796PurposeTo overexpress TIP60 365-513 in Mammalian CellsDepositorInsertTIP60 (KAT5 Human)
UseTags3xFLAGExpressionMammalianMutationaa365-513PromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHB4 gRNA (BRDN0001145313)
Plasmid#76354Purpose3rd generation lentiviral gRNA plasmid targeting human EPHB4DepositorInsertEPHB4 (EPHB4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHB4 gRNA (BRDN0001144879)
Plasmid#76352Purpose3rd generation lentiviral gRNA plasmid targeting human EPHB4DepositorInsertEPHB4 (EPHB4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only