Addgene

Showing: 2341 - 2360 of 3879 results

Results per page:

Sort results by:

mPapaya1-Keratin14-N-18

Plasmid

#56353

Purpose

Localization: Cytokeratin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Keratin14 (KRT14 Human)

Use

Tags

mPapaya1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00119

Plasmid

#34273

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8667 (dimm Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pEBG-hBEN L38&45P

Plasmid

#22182

Depositor

Ananda Roy

Article

Tantin et al J Biol Chem. 2004 Feb 13. 279(7):5460-9.

Insert

hBEN (GTF2IRD1 Human)

Use

Tags

GST and His

Expression

Mammalian

Mutation

Changed Leucine 38 to Proline and changed Leucine…

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pEBB-GFP-hBEN L38&45P

Plasmid

#22183

Depositor

Ananda Roy

Article

Tantin et al J Biol Chem. 2004 Feb 13. 279(7):5460-9.

Insert

hBEN (GTF2IRD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Luecine 38 to Proline and Leucine 45 to P…

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-IFT27

Plasmid

#218722

Purpose

Expresses N-terminally EGFP-tagged IFT27 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT27 (IFT27 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-lyso-pHluorin

Plasmid

#70113

Purpose

Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHluorin (Cd63 Mouse, Aequorea victoria)

Use

Tags

HA-Tag and pHluorin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPN1-ATG9A

Plasmid

#198529

Purpose

Expression of ATG9A-GFP fusion protein in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

ATG9A (ATG9A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

silent mutations in codons 4 and 775

Promoter

CMV

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH_blast_MCS_Nard_GFP_LAMIN

Plasmid

#167340

Purpose

Expresses GFP fused to lamin A (LA) in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TetO-Ascl1-puro

Plasmid

#97329

Purpose

Generating lentiviruses to express Ascl1 and puromycin resistance

Depositor

Marius Wernig

Article

Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TET-ON

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
hRAP-pEZT-BM

Plasmid

#177986

Purpose

Expression human RAP containing a C-terminal 6xHis tag in mammalian cells

Depositor

Lora Hooper

Article

Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

Insert

LDL receptor related protein associated protein 1 (LRPAP1 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

Plasmid

#171019

Purpose

Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

LargeBit-SNAPtag- Beta-actin

Use

Lentiviral and Luciferase

Tags

Beta actin and LargeBiT

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pmScarlet_Giantin_C1

Plasmid

#85048

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Use

Tags

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Fv-hCaspase 8-2A-GFP

Plasmid

#82712

Purpose

express human caspase 8 in mammalian cells

Depositor

Douglas Green

Article

Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

Insert

Fv-Caspase 8-2A-GFP (CASP8 Human)

Use

Retroviral

Tags

FKBP dimerization domain

Expression

Mammalian

Mutation

deleted amino acids 1-215 of caspase 8

Promoter

LTR

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 CB1

Plasmid

#13391

Purpose

Expresses mouse cannabinoid receptor 1 (CB1) in mammalian cells

Depositor

Mary Abood

Article

Abood et al Biochem Pharmacol. 1997 Jan 24. 53(2):20

Insert

CB1 (Cnr1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
p6345 MSCV-CMV-Flag-HA-Brd4 1-722

Plasmid

#31352

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 1-722 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

*see comments

Promoter

Available since

Oct. 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IB-TMEM192-mKeima

Plasmid

#215359

Purpose

Probe to evaluate lysophagic activity

Depositor

Tamotsu Yoshimori

Article

Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

Insert

transmembrane protein 192 (TMEM192 Human)

Use

Retroviral

Tags

mKeima

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MSP10-bio

Plasmid

#47719

Purpose

Expresses enzymatically monobiotinylated full-length MSP10 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised MSP10

Use

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB2-hPER1-mScarlet-I-CD4-bla

Plasmid

#189980

Purpose

Donor Vector to integrate mScarlet-I C-terminal into the human PER1 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

mScarlet-I

Use

CRISPR

Tags

3xFlag

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCriprV2-sgRNA-PER1-#1

Plasmid

#189987

Purpose

Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Period1 (PER1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mTFP1-Caveolin-C-10

Plasmid

#55468

Purpose

Localization: Membrane, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

NM_001753 (CAV1 Human)

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge