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Lenti‐Cas9‐2A‐Blast

Plasmid

#73310

Purpose

Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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CS-TRE-c-Myc-Ubc-rtTA

Plasmid

#198190

Purpose

Generation of Expandable megakaryocyte cell lines from human iPS cells

Depositor

Hiromitsu Nakauchi

Article

Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

Insert

hC-MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO (pEHA1339)

Plasmid

#209088

Purpose

Backbone plasmid for Tet Responsive transgene expression, FRT integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti TMEM30A

Plasmid

#171789

Purpose

transfer plasmid for lentiviral vector production expressing TMEM30A

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

TMEM30A (TMEM30A Human)

Use

Lentiviral

Tags

6X His + Flag

Expression

Mutation

Promoter

CMV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-extGluc-mKate-NGFR

Plasmid

#104831

Purpose

extGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detection

Depositor

Irmela Jeremias

Article

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

mKate
NGFR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Halo-Tag-128-425-Akap9

Plasmid

#196873

Purpose

Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from Golgi

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Halo-Tag-Akap9 (128-425) (Akap9 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-td-mCherry-Cdk5rap2-CTD

Plasmid

#196854

Purpose

Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

td-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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hV1aR-GFP

Plasmid

#67846

Purpose

mammalian expression of human V1a receptor with GFP tag

Depositor

Christian Gruber

Article

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

V1a receptor (AVPR1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
hV1b-GFP-1

Plasmid

#67847

Purpose

mammalian expression of human V1b receptor with GFP tag

Depositor

Christian Gruber

Article

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

V1b receptor (AVPR1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3_ATP10B

Plasmid

#203695

Purpose

expresses ATP10B

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v1 IQSEC1 WT

Plasmid

#162016

Purpose

Expression of IQSEC1 splice variant 1

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v2 IQSEC1 WT

Plasmid

#162017

Purpose

Expression of IQSEC1 splice variant 2

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-extGluc-NGFR

Plasmid

#104832

Purpose

extGluc luciferase for invivo Imaging, NGFR for flourescent detection or separation

Depositor

Irmela Jeremias

Article

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

extGLuc
NGFR

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v3 IQSEC1 WT

Plasmid

#162018

Purpose

Expression of IQSEC1 splice variant 3

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v4 IQSEC1 WT

Plasmid

#162019

Purpose

Expression of IQSEC1 splice variant 4

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-IQSEC1 v2-E620K GEF dead mutant

Plasmid

#162020

Purpose

Expression of GEF dead IQSEC1 mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Tags

Expression

Mutation

EK

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBactin-AcGFP-Cdk5rap2-CTD

Plasmid

#196853

Purpose

Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

AcGFP-Cdk5rap2-CTD (Cdk5rap2 Rat)

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-PGK-Chst3

Plasmid

#193024

Purpose

AAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoter

Depositor

Bart Nieuwenhuis

Article

Yang et al Mol Psychiatry. 2021 Jul 16. pii: 10.103

Insert

Chst3 (Chst3 Mouse)

Use

AAV

Tags

3xFLAG and myc-tag

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBactin-td-mCherry-Cdk5rap2

Plasmid

#196859

Purpose

Expression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

td-mCherry-Cdk5rap2 (Cdk5rap2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFV2-mVenus

Plasmid

#185653

Purpose

NDUFV2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFV2 (NDUFV2 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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