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Plasmid#155303PurposeSARS-CoV-2 fluorescent reporter 1DepositorInsertSuperfolder GFP fused with SARS-CoV-F1 (ORF1ab Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBF_HsPepT2
Plasmid#73944PurposeXenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tagDepositorInsertPepT2 (SLC15A2 Human)
UseMake rna for xenopus oocyte injectionTagsFlagExpressionMutationPromoterbeta globinAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv1
Plasmid#194645PurposepSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA
Plasmid#82702PurposeAAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.DepositorInsertH1/TO Empty gRNA Expression Cassette
UseAAV and CRISPRTagsCMV promoter driving expression of TetR-P2A-GFP-K…ExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_eZ3_Gal4VP64
Plasmid#169917PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralTagsExpressionMammalianMutationAdditional EGF repeat between the extracellular d…PromoterPGKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
dimSIIN
Plasmid#185661PurposeshApc-expressing lentiviruses with varius levels of expression of SIINFEKLDepositorInsertsmScarlet
miR-30a
shApc
GFP
UseLentiviralTagsExpressionMutationPromoterEf1aAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-LAT Y6F YYYY-LAT YYYY -mCitrine (XSB588)
Plasmid#78516PurposeLentiviral vector for expressing LAT-Y6F YYYY-LAT-YYYY-mCitrine (3x2 Grb2 binding sites)DepositorInsertLAT (LAT Human)
UseLentiviralTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK2 Q207D
Plasmid#80871Purposemammalian expression of ALK2 Q207DDepositorInsertALK2 (ACVR1 Human)
UseTagsHAExpressionMammalianMutationQ207DPromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-3XFLAG
Plasmid#105784PurposeExpression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-3XFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-BirA-FLAG
Plasmid#105786PurposeExpression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-BirA-FLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human b4 nicotinic receptor subunit EM construct
Plasmid#163961PurposepEZT-BM vector with beta4 gene after CMV promoter, published EM constructDepositorInsertHuman beta4 nicotinic acetylcholine receptor subunit EM construct
UseBaculovirus (bacmam) production and mammalian exp…TagsStrepII tag and apocytochrome b(562)RIL (BRIL)ExpressionMammalianMutationPro341-Ser395 replaced with apocytochrome b(562)R…PromoterCMVAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-saBE3.9max C-terminal
Plasmid#137180PurposeExpresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoterDepositorInsertNpu-saBE3.9max_C-terminal
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-saBE3.9max N-terminal
Plasmid#137179PurposeExpresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoterDepositorInsertNpu-saBE3.9max_N-terminal
UseTagsExpressionMammalianMutationCas9 D10APromoterCMVAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-human LRRC33
Plasmid#111602PurposeExpress human LRRC33 in 293T cellsDepositorInserthuman LRRC33 (NRROS Human)
UseTagsmyc/his tagExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDR1-GFP
Plasmid#111657PurposeC-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1-GFP (Bicdl1 Mouse)
UseTags8xHis-tag, GFP, TEV site, and ZZExpressionInsectMutationOptimised for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3xFLAG-hTCAB1-puroR
Plasmid#167459Purpose3rd-gen lentiviral expression of FLAG-TCAB1 (WDR79) in mammalian cellsDepositorInsertTCAB1 (WRAP53 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_V5-ANKRD52
Plasmid#92012PurposeExpresses V5-ANKRD52DepositorInsertANKRD52 (ANKRD52 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAQ_G-alpha
Plasmid#109809PurposeProtein expression and purification of GNAQ_G-alphaDepositorInsertGNAQ_G-alpha (GNAQ Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe JunD-HA neo
Plasmid#58486Purposemammalian expression of mouse JunDDepositorInsertJunD (Jund Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH-eCFP
Plasmid#176555PurposeExpression of eCFP for auxotrophic selection in the absence of histidineDepositorInserteCFP
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-eCFP
Plasmid#176556PurposeExpression of eCFP for auxotrophic selection in the absence of leucineDepositorInserteCFP
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-eCFP
Plasmid#176557PurposeExpression of eCFP for auxotrophic selection in the absence of uracilDepositorInserteCFP
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-mEGFP-TEV
Plasmid#105777PurposeExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).DepositorTypeEmpty backboneUseFlp-in competentTagsmEGFP-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MPRA-MluI-SpeI-EcoRI
Plasmid#190196PurposeEmpty vector for inserting MPRA libraries into AAVDepositorTypeEmpty backboneUseAAV; Massively parallel reporter assay (mpra)TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B3
Plasmid#103004Purposenon-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B3 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU-mRuby2
Plasmid#176549PurposeExpression of mRuby2 for auxotrophic selection in the absence of uracilDepositorInsertyomRuby2
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-mRuby2
Plasmid#176547PurposeExpression of mRuby2 for auxotrophic selection in the absence of histidineDepositorInsertyomRuby2
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
loSIIN
Plasmid#185663PurposeshApc-expressing lentiviruses with varius levels of expression of SIINFEKLDepositorInsertsmScarlet
miR-30a
shApc
GFP
UseLentiviralTagsExpressionMutationPromoterEf1aAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B2
Plasmid#103003Purposenon-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B2 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN-T2dMFot
Plasmid#44558DepositorInsertsPTETREG promoter
rtetR-M2::FFF
UseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationCompared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…PromoterPTETREGAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cdt1ΔPIP-mCherry-Puro
Plasmid#193765PurposeDoxycyline-inducible (TetOn) human Cdt1ΔPIP (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertCdt1ΔPIP-mCherry (CDT1 Human)
UseLentiviralTagsmCherryExpressionMutationPIP degron removed (a.a.1-19 removed)PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_THRA_Hormone-recep
Plasmid#109810PurposeProtein expression and purification of THRA_Hormone-recepDepositorInsertTHRA_Hormone-recep (THRA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-JAK1P1084A-VSV
Plasmid#139358PurposeExpression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)DepositorInsertJAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
UseTagsVSVExpressionMammalianMutationP1084APromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-Myc-CRBN-pGK-HYG
Plasmid#107376PurposeLentiviral expression of myc-CRBNDepositorInsertcereblon (CRBN Human)
UseLentiviralTagsMycExpressionMammalianMutationPromoterUbcPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
tdKatushka2-CENPB-N-22
Plasmid#56033PurposeLocalization: Nucleus/Centromeres, Excitation: 586, Emission: 632DepositorInsertCENPB (CENPB Human)
UseTagstdKatushka2ExpressionMammalianMutationaa 1-169 of CENPB (DNA Binding Domain)PromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_DYNLL1_Dynein-light
Plasmid#110006PurposeProtein expression and purification of DYNLL1_Dynein-lightDepositorInsertDYNLL1_Dynein-light (DYNLL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
IL2 F42A pAcGP67a
Plasmid#41807DepositorInsertIL2 (IL2 Human)
UseTags6x HisExpressionInsectMutationPhenylalanine 42 to AlaninePromoterPolyhedronAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103_AURKB_Pkinase
Plasmid#110025PurposeProtein expression and purification of AURKB_PkinaseDepositorInsertAURKB_Pkinase (AURKB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only