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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,409 results for: ttl

Showing: 2321 - 2340 of 3409 results

  2. pJYDN3

    Plasmid
    #162456
    Purpose
    Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162456-plasmid-map-sequence-id-321464

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  3. FRP2374

    Plasmid
    #127579
    Purpose
    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1
    Insert
    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    127579-plasmid-map-sequence-id-252227

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  4. FRP2372

    Plasmid
    #127578
    Purpose
    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection marker
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1
    Insert
    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    127578-plasmid-map-sequence-id-252226

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  6. pJYDN2

    Plasmid
    #162454
    Purpose
    Yeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA nanobody and Ha and myc
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162454-plasmid-map-sequence-id-321461

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  7. pJYDN2p

    Plasmid
    #162455
    Purpose
    Positive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-DnbALFA (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA nanobody and HA and myc
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162455-plasmid-map-sequence-id-321462

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  12. pBUN276

    Plasmid
    #60673
    Purpose
    overexpression of M. tuberculosis ddl gene in Mycobacterium
    Depositor
    Raul Barletta
    Article
    Feng et al Antimicrob Agents Chemother. 2003 Jan;47
    Insert
    ddl
    Use
    E.coli-mycobacterium shuttle vector
    Tags
    6 codons of M. bovis BCG hsp60
    Expression
    Mutation
    Promoter
    BCG Hsp60
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site

    Plasmid
    #228633
    Purpose
    Bacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Enterovirus Coxsackievirus A71 2A protease
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    C110A
    Promoter
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228633-plasmid-map-sequence-id-464103

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  15. pJB72

    Plasmid
    #226312
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with Y to A CsoS2 MR1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y300A
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226312-plasmid-map-sequence-id-449924

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  16. pJB44

    Plasmid
    #226303
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with C to S MR1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C292S, C310S
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226303-plasmid-map-sequence-id-449915

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  17. pJB43

    Plasmid
    #226302
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    WT CsoS2, frameshift site intact
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226302-plasmid-map-sequence-id-449914

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  20. pYPQ301

    Plasmid
    #205415
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Hs1Cas12a without promoter
    Depositor
    Yiping Qi
    Article
    Li et al Front Genome Ed. 2023 Oct 12;5:1251903.
    Insert
    Hs1Cas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    205415-plasmid-map-sequence-id-414100

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Showing: 2321 - 2340 of 3409 results