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Plasmid#126971PurposeLevel 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only
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pXD69m1-TetW
Plasmid#191246PurposeE. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lentaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MK1274
Plasmid#71431PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…PromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST-Ki
Plasmid#44563Purposefor integrative constitutive expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOCC82
Plasmid#118862Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant proteinDepositorInsertNcoI-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptac-sfGFP
Plasmid#139934PurposeIPTG-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertLacI-Ptac-sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEINO5
Plasmid#179489PurposeMulti-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugationDepositorInsertaacC1
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJYDNgp
Plasmid#162453PurposePositive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.DepositorInsertAga2p-2Glinker-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsHA nad myc and eUnaG2 fluorescent proteinExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMGA-plux-sfGFP
Plasmid#139936PurposeAHL-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertLuxR-Plux-sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 hNLRP3-mNG-STOP
Plasmid#218644PurposeGateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)DepositorInsertNLRP3 (NLRP3 Human)
UseGateway entryTagsmNeonGreenExpressionMutationPromoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-iRFPBb
Plasmid#118237PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven iRFPDepositorInsertflagellin promoter driven iRFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCL-ReDNAP (Y427A)
Plasmid#52346PurposeCEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)DepositorInsertRecoded TP-DNAP
UseTagsExpressionBacterial and YeastMutationPromoterREV1Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMZ2007
Plasmid#204399PurposeA 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsquorum sensing regulator LuxR
GFP
UseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PLux0002Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-mtrC
Plasmid#174615PurposeBacterial expression of mtrC from Shewanella oneidensis MR-1.DepositorInsertMtrC
UseSynthetic Biology; Shuttle vector in e. coli and …TagsExpressionBacterialMutationPromoterpLacIAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_PflaB-mCherryBb
Plasmid#118243PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherryDepositorInsertflagellin promoter driven mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-mEGFPBb
Plasmid#118234PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFPDepositorInsertflagellin promoter driven mEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
LDB107
Plasmid#185423PurposeNUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporinDepositorInsertNUP1
UseTagsHAExpressionYeastMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-mCitrineBb
Plasmid#118236PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCitrineDepositorInsertflagellin promoter driven mCitrine
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_P0826-mCherryBb
Plasmid#118242PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0826 promoter driven mCherryDepositorInsertbb0826 promoter driven mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-msfCFPBb
Plasmid#118232PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfCFPDepositorInsertflagellin promoter driven msfCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only