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Plasmid#101164PurposeE. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only
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pXD70LacZ3-Pcsd-LacZ
Plasmid#191619PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcsd promoter-lacZ, E. lenta constitutive promoterDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAN334
Plasmid#120814PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)DepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEG R3-L4 #BGV194
Plasmid#48954PurposeFor inserting selection gene between AttR3 and AttL4 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)DepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDGE658
Plasmid#153256PurposeM3 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTG56A -myc
Plasmid#107459PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationG56APromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WF
Plasmid#108337PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY128
Plasmid#127713PurposeLevel 1 yeast shuttle vector for a transcription unit with A - D scars. Clone in with BsaI. Has a high-copy yeast replicon and KanMX selection.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-cat_TU
Plasmid#127051PurposeBasic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61.DepositorInsertPart Cat_TU
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BR
Plasmid#108336PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WF
Plasmid#108333PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BF
Plasmid#108334PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WR
Plasmid#108335PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BF
Plasmid#108338PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WR
Plasmid#108339PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BR
Plasmid#108340PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDS1U-PKS12
Plasmid#70150PurposeFusarium graminearum PKS12 encoded on CEN/ARS yeast shuttle vector with DS1U selection markerDepositorInsertPKS12 (PKS12 Fusarium graminearum)
UseTagsExpressionYeastMutationPromoterCUP1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJB53
Plasmid#226309PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with V(T/S)G to AAA CsoS2 MR1-3
UseTagsExpressionBacterialMutationVTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…PromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2861 (pSpy1C-PgyrA_ffluc)
Plasmid#218511Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPgyrAAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3105 (pSpy1C-PxylS2_ffluc)
Plasmid#218512Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPxylS2Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only