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Plasmid#174458PurposeExpress mCherry with late endosome-targeting sequence in mammalian cellsDepositorInsertmCherry with late endosome-targeting sequence
UseTagssmall GTPase (Rab7)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Dll4ICD-HPC4 pBacPAK8.3
Plasmid#17335DepositorInsertDelta-like 4 (Dll4 Mouse)
UseTagsHPC4ExpressionInsectMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
myc-mTOR (1750-2549 aa)
Plasmid#21747DepositorInsertmTOR (1750-2549) (Mtor Rat)
UseTagsmycExpressionMammalianMutationamino acids 1750-2549PromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9
Plasmid#84245PurposeExpresses direct fusion KRAB-Sp dCas9DepositorInsertsKRAB-tagBFP-Sp dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), HA tag, KRAB, and tagBFPExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc human NDR1
Plasmid#37023DepositorInsertNDR1 (STK38 Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 MT
Plasmid#78261PurposepsiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertmutated let-7 target site
UseLuciferase; Microrna activityTagsExpressionMammalianMutationnucleotides 2-5 and 10-11 changed from CTCC to GA…PromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Str-KDEL_ST-SBP-tagBFP
Plasmid#65266Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
UseTagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQFlag-USP7 WT puroR
Plasmid#46751PurposeRetroviral vector that expresses wild type Flag-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-EGFP
Plasmid#65252Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5
Plasmid#120825PurposeEctopic expression of ZIKV proteinDepositorInsertNS5 ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-GASC1
Plasmid#24214PurposeMammalian expression of human JMJD2C with HA tagDepositorInsertGASC1 (KDM4C Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5-YFP
Plasmid#120831PurposeEctopic expression of ZIKV proteinDepositorInsertZIKV NS5-eYFP
UseTagseYFP-V5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNA500
Plasmid#149576Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-TetR_W43F-3xmNG
Plasmid#174880PurposePiggyBac transposon vector for expression of TetR(W43F)-3x-mNeonGreenDepositorInsertTetR(W43F)-3xmNeonGreen
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tag5Amyc-GSK3b CA
Plasmid#16261DepositorInsertGSK3 beta (GSK3B Human)
UseTagsMycExpressionMammalianMutationConstitutively active: S9APromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/Envelope
Plasmid#120821PurposeEctopic expression of ZIKV proteinDepositorInsertEnvelope ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
DsRed-Rab5 WT
Plasmid#13050DepositorInsertRAB5A (Canis familiaris) (RAB5A Canis familiaris)
UseTagsDsRed2 and HAExpressionMammalianMutationasparagine 3 changed to serine (neutral); serine …PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
phage ubc nls ha pcp gfp
Plasmid#64539PurposeUsed to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.DepositorInsertPCP
UseLentiviralTagsEGFP, FactorXa site, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-FL
Plasmid#190821PurposeFor expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags6x HisExpressionBacterialMutationCodon optimized for expression in E.coliPromoterT7Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits