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pGSTag BimEL (3SA)

Plasmid

#23100

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (3SA) (Bcl2l11 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

changed Serine 55/65/73 to alanine

Promoter

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pBacPAK-HFT-dKDM4A-H195A

Plasmid

#20236

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Use

Tags

FLAG, His, and TEV

Expression

Insect

Mutation

changed His 195 to Ala

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
Neurl1-Flag pBacPAK8-3

Plasmid

#17322

Depositor

Ronald Conaway, Joan Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

Tags

FLAG

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
NHR1-Flag pBacPAK8-3

Plasmid

#17323

Depositor

Ronald Conaway, Joan Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

Tags

FLAG

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
NHR1+2-Flag pBacPAK8-3

Plasmid

#17324

Depositor

Ronald Conaway, Joan Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

Tags

FLAG

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
Cdk12_intron3_sg2_pX330

Plasmid

#127174

Purpose

sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6 Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron4_sg1_pX458

Plasmid

#127175

Purpose

sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 4 sgRNA

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6 Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PARP1-3xFlag-WT

Plasmid

#111575

Purpose

Expresses full length PARP1 in mammalian cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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Tag5Amyc-GSK3b WT

Plasmid

#16260

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

GSK3 beta (GSK3B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Tag5Amyc-GSK3b CA

Plasmid

#16261

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

GSK3 beta (GSK3B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Constitutively active: S9A

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2/Capsid

Plasmid

#120816

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

Capsid ZIKV

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 WT

Plasmid

#69003

Purpose

mammalian expression of wild type human p53

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Human V5-IRF3-pcDNA3

Plasmid

#32713

Depositor

Saumen Sarkar

Article

Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

Insert

IRF3 (IRF3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-dCas9-TurboID-blast

Plasmid

#222599

Purpose

Express fussed dCas9-TurboID for proximity labeling

Depositor

Yarui Diao

Article

Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

Insert

dCas9-TurboID

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1α

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHsp83-NLS-HA-2xPCP-2xGFP

Plasmid

#71243

Purpose

Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

2xPCP-2xGFP

Use

P element-mediated germline transformation

Tags

EGFP

Expression

Insect

Mutation

Promoter

Hsp83

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-ncOGT-FL

Plasmid

#190821

Purpose

For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

O-GlcNAc Transferase (OGT Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

Codon optimized for expression in E.coli

Promoter

T7

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Str-KDEL_TNF-SBP-mCherry

Plasmid

#65279

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mCherry-MHC-IIA

Plasmid

#35687

Depositor

Venkaiah Betapudi

Article

Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

Insert

MHC IIA (MYH9 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD70LacZ6-LacI-Ptac(LacO)-LacZ

Plasmid

#191621

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-Rictor corrected

Plasmid

#11367

Purpose

Mammalian expression of myc tagged human RICTOR

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge