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AAVS1-Puro-CAG-GCaMP6s

Plasmid

#120896

Purpose

Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

Depositor

Xiaojun Lian

Article

Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

Insert

GCaMP6s

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG FUCCI

Plasmid

#136934

Purpose

donor plasmid for constitutive FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Use

Synthetic Biology

Tags

Clover and mKO2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

Plasmid

#62956

Purpose

Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-PKCa-C1

Plasmid

#112264

Purpose

ITRACKalpha Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase C alpha (PRKCA Bovine)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

A11V (please see depositor comments below)

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-PKCa-mEGFP-N1

Plasmid

#112269

Purpose

IDOCKSalpha Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

Protein Kinase C alpha (PRKCA Bovine)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

A11V (please see depositor comments below)

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-Blasticidin-CAG-Flpe-ERT2

Plasmid

#68461

Purpose

AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

mammalian-codon-optimized Flpe recombinase fused with ERT2

Use

Synthetic Biology

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

Plasmid

#89991

Purpose

Donor template for generation of SOX2-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

none

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_KDM1A

Plasmid

#86260

Purpose

Donor vector for 3' FLAG tag of human KDM1A

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KDM1A homology arms (KDM1A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMIA10.5-Clover

Plasmid

#214449

Purpose

BxbI landing-pad mClover donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

mClover

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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p15_attB(bxb)_lox

Plasmid

#183764

Purpose

Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-trGluc

Plasmid

#158959

Purpose

DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

trGluc

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-Pur-CAG-hM4Di-mCherry

Plasmid

#80947

Purpose

human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Insert

hM4Di-mCherry

Use

Tags

mcherry fusion

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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AAVS1-Pur-CAG-hM3Dq-mCherry

Plasmid

#80948

Purpose

human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Insert

hM3Dq-mCherry

Use

Tags

mcherry fusion

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pBR_attB(C31)_FRT

Plasmid

#183761

Purpose

phiC31-specific donor plasmid for the cloning of payloads <20 kb

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #3

Plasmid

#139659

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-iPEmax-Hygro

Plasmid

#214020

Purpose

inducible PEmax system for controllable prime editing; this plasmid is used to insert PEmax prime editor in one allele of AAVS1 locus

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

PEmax

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

Plasmid

#154826

Purpose

Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

mCherry
NeoR
ZsGreen1-DR

Use

CRISPR and Cre/Lox

Tags

PEST

Expression

Mammalian

Mutation

Promoter

CMV, EF1a, and SV40 early promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Bxb1_miRFP703

Plasmid

#183759

Purpose

Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

miRFP703

Use

Tags

Lck lipidation signal

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-CREB

Plasmid

#137001

Purpose

mEGFP-CREB donor for G-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00044

Plasmid

#34206

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17117 (hth Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only