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Plasmid#108891PurposeMultisite gateway vector for 3' tagging with a conditionally stable nanobody against GFPDepositorInsertcsGBP
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P)
Plasmid#185800Purposeone plasmid encoding scFv GFP and pcp-5xSunTag components for SunRISER SRv1DepositorInsertcmv-sfGFP-GB1-scAB--ubc-nls-pcp-5xSunTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB_init-Sb44
Plasmid#159421PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44DepositorInsertSb44
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB_init_Sb68
Plasmid#159423PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68DepositorInsertSb68
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
LaM8-GG15
Plasmid#159593PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at GG15
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-T1
Plasmid#190825PurposeFor mammalian expression of T1, the RNA aptamer of ncOGT. Expression is driven by a U6 promoter.DepositorInsertT1 (an RNA aptamer of O-GlcNAc Transferase)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB_init_Sb92
Plasmid#159424PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92DepositorInsertSb92
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP
Plasmid#194934PurposeLV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb2E7
Plasmid#184681PurposeNIR-Fb to HIV gp41DepositorInsertNIR-Fb2E7
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-bc339
Plasmid#190848PurposeFor mammalian expression of bc339, the RNA aptamer of β-catenin. Expression is driven by a U6 promoter.DepositorInsertbc339 (an RNA aptamer of β-catenin)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-AP3
Plasmid#190826PurposeFor mammalian expression of AP3, the RNA aptamer of GFP. Expression is driven by a U6 promoter.DepositorInsertAP3 (an RNA aptamer of GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
p3E-csChBP
Plasmid#108893PurposeMultisite gateway vector for 3' tagging with a conditionally stable nanobody against mCherryDepositorInsertdChBP
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceJune 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag VP64 gRNA4 (FWA)
Plasmid#120249PurposeCRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNADepositorInsertg4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB_init-Sb66
Plasmid#159422PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66DepositorInsertSb66
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A-APEX2-ChBP
Plasmid#108879PurposeExpression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)DepositorInsertEGFP-P2A-APEX2-ChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
5aa SunTag VP64 EVD
Plasmid#115483PurposeCRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb4EIG
Plasmid#184683PurposeNIR-Fb to E.coli dihydrofolate reductaseDepositorInsertNIR-Fb4EIG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd gRNA4 (FWA)
Plasmid#115486PurposeCRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNADepositorInsertg4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb59H10
Plasmid#184682PurposeNIR-Fb to HIV p24DepositorInsertNIR-Fb59H10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-7D12Gln13TAG
Plasmid#125264PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsertGln13TAG 7D12
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only