-
Plasmid#63790PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection markerDepositorInserteSapphire-Twin-Strep tag followed by URA3
UseE. coli cloning vectorTagseSapphire-Twin-Strep tagExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Supervillin-45
Plasmid#57671PurposeLocalization: Membrane/Actin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertSupervillin
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-22HT-MSI1(7-192)Q30D
Plasmid#60346PurposeQ30D mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationQ30D; contains amino acids 7-192 of Msi1PromoterT7Available sinceDec. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R53E
Plasmid#60352PurposeR53E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR53E; contains amino acids 7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)G64A
Plasmid#60351PurposeG64A mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationG64A; contains amino acids 7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)H83L
Plasmid#60350PurposeH83L mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationH83L; contains amino acids 7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)G35D
Plasmid#60347PurposeG35D mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationG35D; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)G35E
Plasmid#60348PurposeG35E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationG35E; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)H83F
Plasmid#60349PurposeH83F mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationH83F; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab18 WT
Plasmid#58453Purposeexpression of drosophila YFP Rab18DepositorInsertRab18 (Rab18 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K88A
Plasmid#58402PurposeExpresses murine IFITM3-K88A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysine 88 to AlaninePromoterCMV IEAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K104A
Plasmid#58403PurposeExpresses murine IFITM3-K104A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysine 104 to AlaninePromoterCMV IEAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab19 WT
Plasmid#58454Purposeexpression of drosophila YFP Rab19DepositorInsertRab19 (Rab19 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab2 DN
Plasmid#58445Purposeexpression of drosophila YFP-Rab2 dominant negative mutantDepositorInsertRab2 (Rab2 Fly)
UseTagsYFPExpressionInsectMutationS20N (dominant negative)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab2 CA
Plasmid#58443Purposeexpression of drosophila YFP-Rab2 constitutively active mutantDepositorInsertRab2 (Rab2 Fly)
UseTagsYFPExpressionInsectMutationQ65L (constitutive active)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-hSH3BP4
Plasmid#46895Purposeexpression of shRNA against human SH3BP4DepositorInsertSH3BP4 (SH3BP4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationW92A (mutated SH3 domain)PromoterAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-mSH3BP4
Plasmid#46894Purposeexpression of shRNA against mouse SH3BP4DepositorInsertSH3BP4 (Sh3bp4 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationW92A (mutated SH3 domain)PromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-851 A50I mutation
Plasmid#46174DepositorInsertVcl 1-851 A50I (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 1-851 A50I mutation (talin binding domain mut…PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab14 DN
Plasmid#37715DepositorInsertRab14 (Rab14 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS49N (dominant negative)PromoterGAL4Available sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab26 DN
Plasmid#37730DepositorInsertRab26 (Rab26 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT204N (dominant negative)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab19 CA
Plasmid#37722DepositorInsertRab19 (Rab19 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ80L (constitutive active)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab19 DN
Plasmid#37721DepositorInsertRab19 (Rab19 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT35N (dominant negative)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab19 WT
Plasmid#37720DepositorInsertRab19 (Rab19 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP RabX1 DN
Plasmid#37751DepositorInsertRabX1 (chrw Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT19N (dominant negative)PromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP RabX1 CA
Plasmid#37752DepositorInsertRabX1 (chrw Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ66L (constitutive active)PromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCaShs-hopscotch
Plasmid#37299DepositorInserthopscotch (hop Fly)
UseTagsExpressionInsectMutationPromoterHsp70Available sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-MRPL15
Plasmid#31345DepositorInsertmitochondrial ribosomal protein L15 mature form (MRPL15 Human)
UseTagsHisExpressionBacterialMutationHas the amino acids in the predicted mature form …PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBS MIP2gamma
Plasmid#19224DepositorInsertJun-suppressed chemokine (CXCL14 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAcUW51-CgE
Plasmid#13762DepositorInsertHSV-1 glycoprotein E
UseTagsHisExpressionInsectMutationC-terminal region of the HSV-1 gE (KOS strain ect…PromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-A'Q-mito
Plasmid#158766PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-'Q-mito
Plasmid#158767PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-'S-mito
Plasmid#158768PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
eeBxb1
Plasmid#222339PurposePlasmid for expressing evolved and engineered Bxb1 in the mammalian cellsDepositorInsertCodon-optimized evolved and engineered Bxb1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-pA-Hia5
Plasmid#174372PurposePlasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertprotein A - hia5
UseTags6xHis and Protein AExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SEP-Glur2 (MCA10)
Plasmid#64941Purposemammalian expression of rat GluR2DepositorInsertGluR2 (Gria2 Rat)
UseTagsSEP (superecliptic pHluorin)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
BPK1520
Plasmid#65777PurposeHuman expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNADepositorInsertSpCas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4)P Probe
Plasmid#211509PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteriaDepositorInsertSidC (P4C)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4,5)P2 Probe
Plasmid#211513PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteriaDepositorInsertPLCdelta1-PH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,5)P2 Probe
Plasmid#211512PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteriaDepositorInsertSnxA-2xPX
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST
Plasmid#127332PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRESDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
evoBxb1
Plasmid#222338PurposePlasmid for expressing evolved Bxb1 in the mammalian cellsDepositorInsertCodon-optimized evolved Bxb1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
wtBxb1
Plasmid#222337PurposePlasmid for expressing wild type Bxb1 in the mammalian cellsDepositorInsertCodon-optimized wild type Bxb1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4)P2 Probe
Plasmid#211511PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteriaDepositorInsertTAPP1 cPHx3
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4,5)P3 Probe
Plasmid#211514PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteriaDepositorInsertBTK1-PH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
HDAC8 Flag
Plasmid#13825DepositorInsertHDAC8 (HDAC8 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Electra2-P2A-EGFP
Plasmid#184936PurposeExpresses blue fluorescent protein Electra2 and EGFP in mammalian cellsDepositorInsertElectra2-P2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mTagBFP2-WPRE
Plasmid#193339PurposeAAV-mediated expression of mTagBFP2 under the TRE promoter in mammalian cellsDepositorInsertmTagBFP2
UseAAVTagsExpressionMammalianMutationA XhoI site in the coding sequence is silencedPromoterTREAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
SB100X in pCAG globin pA
Plasmid#127909PurposeSleeping Beauty Transposase expression vector driven by the CAG promoterDepositorInsertSleeping Beauty Transposase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir
Plasmid#90004PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]DepositorInsertsFKBP-linker-Myc-NBirA*
CBirA*-linker-FRB
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only