Addgene

We narrowed to 4,144 results for: Pet2;

Showing: 3481 - 3510 of 4144 results

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pBEL1308

Plasmid

#155146

Purpose

Expresses MlaFB, His tag on MlaB C-term

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(MlaB-6xHis)

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1957

Plasmid

#155151

Purpose

Expresses Strep-MlaF-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

added Streptavidin tag on MlaF N-terminus

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2074

Plasmid

#155155

Purpose

Expresses Strep-MlaF-(6xHis-TEV-MlaB_T52E)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaB_T52E

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2076

Plasmid

#155156

Purpose

Expresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF_K47A

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2077

Plasmid

#155157

Purpose

Expresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF_E170Q

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2078

Plasmid

#155158

Purpose

Expresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF_Y261A

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2079

Plasmid

#155159

Purpose

Expresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF_L265A

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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R2x2_VAL88

Plasmid

#160761

Purpose

Expresses R2x2_VAL88 in bacterial cells

Depositor

Nobuyasu Koga

Article

Koga et al Proc Natl Acad Sci U S A. 2020 Nov 23. p

Insert

R2x2_VAL88

Use

Tags

6xHis

Expression

Bacterial

Mutation

10-residue mutations of Rsmn2x2_5_6

Promoter

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1996

Plasmid

#139900

Purpose

Expression plasmid for LetB PLL4 deletion (without TM region)

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta459-360)-6xHis

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2045

Plasmid

#139924

Purpose

Expression plasmid for 4 ring (rings 1347) LetB (without TM region) mutant

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB (43-159; 280-515; 747-877)

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2044

Plasmid

#139923

Purpose

Expression plasmid for 4 ring (rings 1237) LetB (without TM region) mutant

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB (43-391; 747-877)

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1998

Plasmid

#139902

Purpose

Expression plasmid for LetB PLL6 deletion (without TM region)

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta703-708, Q702G, E709G)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1997

Plasmid

#139901

Purpose

Expression plasmid for LetB PLL5 deletion (without TM region)

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta581-590, K580G, Q591G)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1995

Plasmid

#139899

Purpose

Expression plasmid for LetB PLL3 deletion (without TM region)

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta347-360, P346G)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2002

Plasmid

#139887

Purpose

Expression plasmid for LetAB with 4 ring (rings 1567) LetB (without TM region) mutant

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta161-514)-6xHis

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2001

Plasmid

#139886

Purpose

Expression plasmid for LetAB with 3 ring (rings 167) LetB (without TM region) mutant

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta161-634)-6xHis

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1755

Plasmid

#139879

Purpose

Expression plasmid for naturally occurring 8xMCE protein (without TM region)

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

Denitrovibrio_acetiphilus_Dacet_0211

Use

Tags

Expression

Bacterial

Mutation

Denitrovibrio_acetiphilus_Dacet_0211(41-988)-6xHis

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1753

Plasmid

#139878

Purpose

Expression plasmid for naturally occurring 6xMCE protein (without TM region)

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

Pseudomonas_aeruginosa_PA4689

Use

Tags

Expression

Bacterial

Mutation

Pseudomonas_aeruginosa_PA4689(41-768)-6xHis

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1752

Plasmid

#139877

Purpose

Expression plasmid for naturally occurring 5xMCE protein (without TM region)

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

Nitrincola_nitratireducens_D791_00999

Use

Tags

Expression

Bacterial

Mutation

Nitrincola_nitratireducens_D791_00999(47-644)-6xH…

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1751

Plasmid

#139876

Purpose

Expression plasmid for naturally occurring 4xMCE protein (without TM region)

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

Desulfocapsa_sulfexigens_UWK_02604

Use

Tags

Expression

Bacterial

Mutation

Desulfocapsa_sulfexigens_UWK_02604(38-533)-6xHis

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDC24

Plasmid

#131368

Purpose

expression of MEG-3Cterm (aa545-862) in C. elegans

Depositor

Geraldine Seydoux

Article

Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

Insert

meg-3 (aa545-862) (meg-3 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pDC21

Plasmid

#131367

Purpose

expression of MEG-3IDR (aa1-544) in C. elegans

Depositor

Geraldine Seydoux

Article

Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

Insert

meg-3 (aa1-544) (meg-3 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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6CUI (BRD2)

Plasmid

#119963

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

BRD2 (BRD2 Human)

Use

Tags

GST tag followed by a long linker and TEV cleavag…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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6DUB (LOC149281)

Plasmid

#119967

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

LOC149281 (METTL11B Human)

Use

Tags

6xHIS, thrombin,SUMO and TEV cleavage

Expression

Bacterial

Mutation

contains amino acids 58-278

Promoter

T7

Available since

Jan. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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5T1G (CBX1)

Plasmid

#101884

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CBX1 (CBX1 Human)

Use

Tags

6xHis, TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 30, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pET-PF2050

Plasmid

#100721

Purpose

Expression of Pyrococcus furiosus PF2050

Depositor

Byung Il Lee

Article

Han et al FEBS Lett. 2012 May 7;586(9):1384-8. doi

Insert

PF2050

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promotor

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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2QO2 (EPHA3)

Plasmid

#101271

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

EPHA3 (EPHA3 Human)

Use

Tags

Hexahistidine tag with Thrombin cleavage

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 2, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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CTL2

Plasmid

#92212

Purpose

Expresses C-Terminal segment of C. elegans Piezo1 in E. coli

Depositor

Douglas Rees

Article

Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

Insert

CTL2 of PIEZO1

Use

Tags

6x Histidine Tag

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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Ppj219(JBEI-13475)

Plasmid

#89785

Purpose

for expression of N-StrepII-3607

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

3607

Use

Tags

StrepII

Expression

Bacterial

Mutation

Promoter

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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Ppj184 (JBEI-13431)

Plasmid

#89784

Purpose

for expression of N-His-3607

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

3607

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge