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Plasmid#109518PurposeMuscle specific zebrafish expression of mKate2 fused to Caveolin3DepositorInsertCav3 (cav3 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Dnm2a
Plasmid#109519PurposeMuscle specific zebrafish expression of mKate2 fused to Dynamin2aDepositorInsertDnm2a (dnm2a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Dnm2a-DN
Plasmid#109520PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative Dynamin2aDepositorInsertDnm2a (dnm2a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationDNPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTone-gata2aECE-EGFP
Plasmid#132974Purposegata2a endothelial enhancer (x6) and basal promoter driving egfp in pTol1 backboneDepositorInsertseGFP
gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
UseUnspecified; Transposon-mediatedTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSi-check2-hRac2 3'UTR
Plasmid#97160PurposeFor dual luciferase report assayDepositorInsertRAC 3'UTR (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-LC3-RFP-LC3ΔG
Plasmid#168997PurposeExpresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME minprom_creERT2
Plasmid#195966PurposeGateway pME CreERT2 ORFDepositorInsertmouse beta-globin minimal promoter creERT2
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
elavl3:hArr-TEV;he1.1:YFP
Plasmid#218855PurposeD. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.DepositorInserthArr
UseTagshuman Arrestin-Tobbaco Etch Virus protease fusion…ExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Xla.Tubb2:hArr-TEV;he1.1:eYFP
Plasmid#218859PurposeXla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.DepositorInserthArr
UseTagshuman Arrestin-Tobbaco Etch Virus protease fusion…ExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
458 p3E-2A-EGFP-CAAXpA
Plasmid#195968PurposeGateway p3E 2A fluorophoreDepositorInsert2A-EGFP-CAAX (membrane)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_eZ3_Gal4VP64
Plasmid#169917PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralTagsExpressionMammalianMutationAdditional EGF repeat between the extracellular d…PromoterPGKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218205PurposeZebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-H2B-GCaMP6s
Plasmid#59530PurposeExpressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTransposable elementTagsExpressionMutationPromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX
Plasmid#154910Purpose1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.DepositorInsertPhytochrome B (PHYB Synthetic, Mustard Weed)
UseXenopus/avian/zebrafishTagsCAAX membrane moiety and mCherryExpressionMammalianMutation1-621 amino acids of Arabidopsis PHYBPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Y276H PHYB-CAAX
Plasmid#154911Purpose1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.DepositorInsertPhytochrome B (PHYB Mustard Weed)
UseXenopus/avian/zebrafishTagsCAAX membrane moiety and mCherryExpressionMammalianMutation1-621 amino acids of Arabidopsis PHYB, Tyrosine r…PromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK069 desmaMCS:minprom_mApple,exorh:EGFP
Plasmid#195982PurposeExpression constructDepositorInsertdesmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-2C-NLS in pCSDest
Plasmid#126637PurposeExpresses AsCas12a-2C-NLS in mammalian cellsDepositorInsertAsCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha A-crystallin 1kb promoter/AcGFP
Plasmid#98096PurposeaA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha A-crystallin 1 kb fragment (-1028/-1)Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME minprom KalTa4
Plasmid#195964PurposeGateway pME Gal4 variantDepositorInsertmouse beta-globin minimal promoter KalTA4 (Gal4)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only