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Plasmid#205035PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(0)-L3
UseTagsGRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationE16R, I138R, D207R, N222K, L246RPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)-H1
Plasmid#205036PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(0)-H1
UseTagsKRRRKK and MGHHHHHGGAExpressionBacterialMutationE16R, I138R, D207R, N222K, L246RPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)-H2
Plasmid#205037PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(0)-H2
UseTagsKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialMutationE16R, I138R, D207R, N222K, L246RPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)-H3
Plasmid#205038PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(0)-H3
UseTagsKRRRKKKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialMutationE16R, I138R, D207R, N222K, L246RPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-DEWS
Plasmid#208799PurposeXenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationD374A; E377A; W381A; S437APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-Δ24
Plasmid#208801PurposeXenopus laevis His6-Myc-DONSON (1-24aa removed)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationΔ24, deletion of 1-24aaPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-DEW
Plasmid#208802PurposeXenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationD374A; E377A; W381APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-S437A
Plasmid#208803PurposeXenopus laevis His6-Myc-DONSON (S437A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationS437APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-ΔD1
Plasmid#208804PurposeXenopus laevis His6-DONSON (1-155aa removed)DepositorInsertDONSON (donson.L Frog)
UseTagsHisExpressionBacterialMutationΔD1, deletion of 1-155aaPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-TrxF
Plasmid#207454PurposeE. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tagDepositorInsertThioredoxin F
UseTags6xHisExpressionBacterialMutationCodon optimized for E coli expressionPromoterT7Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24-1~93
Plasmid#208633Purposefor E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93DepositorInserthuman RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationonly aa 1~93, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-eIF4E-TwStrep
Plasmid#208638Purposefor E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)DepositorInserthuman eIF4E (ENST00000450253) (EIF4E Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-Twstrep
Plasmid#208623Purposefor E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)DepositorInserthuman RPL3 (ENST00000216146) (RPL3 Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-E3(63)-His6
Plasmid#212717PurposeBacterial expression of NUbo-E3(63)-His6DepositorInsertE3(63)
UseTags6xHis and NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-E3(48)-His6
Plasmid#212714PurposeBacterial expression of NUbo-E3(48)-His6DepositorInsertE3(48)
UseTags6xHis and NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-hXRCC1pd-HIS
Plasmid#206036PurposeBacterial expression of PAR-deficient hXRCC1pd with 6xHis tag, under the control of T7 promoterDepositorInserthXRCC1
UseTagsHISExpressionBacterialMutationS103A,R186A,S184A,S193A,S219A,S220A,S236A,S268APromoterT7Available sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f L27A
Plasmid#204138PurposeProduction of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coliDepositorInsertjGCaMP8f L27A
UseTagsN-terminal His tagExpressionBacterialMutationLeucine 27 changed to AlaninePromoterT7Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-aδNb072
Plasmid#204627PurposeExpresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.DepositorInsertaδNb072
UseTagsHis6 and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSyt1C2AB(80-421)msGFP
Plasmid#209009PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis and msfGFPExpressionBacterialMutationaa 80-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETmsGFPSyt1(80 JuxtaK-142)
Plasmid#209013PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-msfGFPExpressionBacterialMutationaa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…PromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only