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Plasmid#58774PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRG645_lexO-AscI_LexA-TF_LEU2MX
Plasmid#154813PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIHEU_miniSOG2 T2A H3.3A-EGFP
Plasmid#87411PurposeUAS vector to express Histone 3.3A GFP fusion and miniSOG2 in DrosophilaDepositorInsertmini singlet oxygen generator 2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Pur
Plasmid#171683Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertstetracycline-controlled transactivator
Puromycin
EF-1a promoter
UseTagsExpressionMammalianMutationPromoterEF-1a promoter and RPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Neo
Plasmid#171684Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)DepositorInsertNeomycin
UseTagsExpressionMammalianMutationPromoterRPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRG714_lexO-AscI_LexA-TF_URA3MX
Plasmid#154822PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x3
Plasmid#58773PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-psba1-Ppsba2-dCas9-SpR
Plasmid#73220PurposeContains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.DepositorInsertdCas9 from S. pyogenes
UseTagsc-mycExpressionBacterialMutationSilent mutation at bp 1341 A->C to remove an E…PromoterPpsbA2Available sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJP102
Plasmid#176480PurposeExpresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoterDepositorInsertsactin > NatR
EF1 > mScarlet
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJP103
Plasmid#176481PurposeExpresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoterDepositorInsertsactin > HygR
EF1 > mScarlet
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_PTS2
Plasmid#65676PurposeExpresses PTS2-CNL in mammalian cellsDepositorInsertperoxisomal targeting signal 2
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG711_lexO-AscI_LexA-TF_HIS3MX
Plasmid#154820PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_H2B
Plasmid#65702PurposeExpresses YNL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE
Plasmid#140087PurposeBackbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistanceDepositorInsertLbCpf1 (LbCas12a) crRNA backbone
UseLentiviralTagsEF1a-PuroExpressionMammalianMutationPromoterU6Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBDT
Plasmid#62660PurposeTargeting vector for PITT landing pad knock-in into the Rosa26 locusDepositorInsertseGFP
neomycin
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_LAMP1
Plasmid#65686PurposeExpresses LAMP1-ONL in mammalian cellsDepositorInsertLAMP1
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_H2B
Plasmid#65704PurposeExpresses ONL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBDU
Plasmid#62661PurposeTargeting vector for PITT landing pad knock-in into the Rosa26 locusDepositorInsertseGFP
neomycin
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBGV
Plasmid#62664PurposeDonor vector for mCFP knock-in via landing padDepositorInsertmCFP
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_PTS2
Plasmid#65677PurposeExpresses PTS2-ONL in mammalian cellsDepositorInsertperoxisomal targeting signal 2
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only