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Plasmid#185862PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXR003: CasRx gRNA cloning backbone
Plasmid#109053PurposehU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0043-PspCas13b crRNA backbone
Plasmid#103854PurposeFor cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caacDepositorHas ServiceCloning Grade DNAInsertU6-PspCas13b DR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBlu/gRNA
Plasmid#59188PurposeServes as a shuttle vector for target oligo before insertion into Cas9 destination vectorDepositorInsertGuide RNA Cassette
UseCRISPRTagsExpressionPlantMutationPromoterU6 (Arabidopis)Available sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pnCasPA-BEC
Plasmid#113349PurposeA cytidine deaminase-mediated base-editing plasmid in Pseudomonas speciesDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lwa_Cas13a_WT
Plasmid#91865PurposeBacterial expression for Cas13aDepositorInsertLwaCas13a
UseCRISPRTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9_GFP
Plasmid#44719PurposeCo-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cellsDepositorInsertCas9-2A-GFP
UseCRISPRTags2A-GFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
SadCas9 VPR
Plasmid#188514PurposeExpresses FLAG tagged SadCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/N580APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR_011
Plasmid#59702PurposeThis lentiviral vector can be used to assay Cas9 activity.DepositorInsertEGFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCasPA
Plasmid#113347PurposeBacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEn-Chimera
Plasmid#61432PurposeGateway-Entry vector containing AtU6-26 promoter and sgRNA backboneDepositorInsertAtU6-26:sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330A-FBL/PITCh
Plasmid#63671PurposeExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFD4-U6:1_U6:3tandemgRNAs
Plasmid#49411Purposeexpression of two gRNAs from Drosophila U6:1 and U6:3DepositorInsertsdU6-1:gRNA
dU6-3:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6-1 and dU6-3Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXR004: CasRx pre-gRNA cloning backbone
Plasmid#109054PurposehU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFYF1320 EGFP Site#1
Plasmid#47511Purposehuman gRNA expression vector targeting EGFPDepositorInsertgRNA-EGFP site 1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEcCas-2.0
Plasmid#190697PurposeConstitutive expressing of Cas9 and inducible expression of lambda RedDepositorInsertCas9 and Lamda Red system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
DR274
Plasmid#42250Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcriptionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR; Zebrafish expressionTagsExpressionWormMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpdCas9 VPR
Plasmid#188509PurposeExpresses FLAG tagged SpdCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/H840APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0046-EF1a-PspCas13b-NES-HIV
Plasmid#103862PurposeExpresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasmDepositorInsertPspCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCEC-red
Plasmid#196040PurposeAllows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate AssemblyDepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Grna cloningTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only