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pIntegrase_5

Plasmid

#60577

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_5

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV_SARS-CoV-2_Sdel18

Plasmid

#207307

Purpose

Mammalian expression of SARS2 coronavirus S protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SARS-CoV2_S (S SARS2 coronavirus)

Use

Tags

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA_mTurquoise2

Plasmid

#108237

Purpose

Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

PsbA promoter - mTurquoise2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLSHiFiNG-P2A-Puro

Plasmid

#136903

Purpose

Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSHiFiNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Expression

Mutation

R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

Promoter

EF1s

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6 MCV LTco S239A

Plasmid

#112194

Purpose

Expression of MCPyV LT mutant (Ser 239 to Ala)

Depositor

Patrick Moore

Article

Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

Insert

MCPyV LT codon optimized

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-human lyn–GFP

Plasmid

#35958

Purpose

Expresses human lyn protein codon optimized for zebrafish expression.

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

LYN (LYN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

wt

Promoter

cmv

Available since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2+_Flag-myc-NES-Tir1

Plasmid

#117717

Purpose

mRNA synthesis of Flag-myc-NES-TIR1 for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

TRANSPORT INHIBITOR RESPONSE 1

Use

Tags

Flag-Myc-NES

Expression

Mammalian

Mutation

codon optimized TIR1 for murine expression

Promoter

SP6

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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JH04

Plasmid

#73513

Purpose

mRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mRuby2::PH::tbb-2

Use

Tags

mRuby2

Expression

Mutation

Promoter

mex-5

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-SpCAS9-NG-pA

Plasmid

#131465

Purpose

Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Insert

Codon optimized SpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pUB-Cas9

Plasmid

#86556

Purpose

For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

Depositor

Andreas Weber

Article

Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

Insert

Cas9

Use

CRISPR

Tags

Expression

Plant

Mutation

Cas9 is codon optimized for C. reinhardtii

Promoter

UBIQUITIN10

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pTU398

Plasmid

#178980

Purpose

13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

gRAMP

Use

Tags

Strep-Tag II SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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GatA_pET29b

Plasmid

#215406

Purpose

Expression construct for GatA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

GatA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pMXs-neo

Plasmid

#51260

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dgcr8_1

Plasmid

#73525

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pMSP2

Plasmid

#26282

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009 . 464():211-31.

Insert

MSP2

Use

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of dimer of deletion mutant (1-43)…

Promoter

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
Marathon-RT for Split-PE (pEH59)

Plasmid

#190108

Purpose

Expresses Marathon-RT (human codon optimization)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

Marathon-RT

Use

Tags

bpNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUCHR-osTIR1-IRES-GFP

Plasmid

#110661

Purpose

Lentiviral vector for stable expression of osTIR1 and selection with GFP

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

osTIR1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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CH00X

Plasmid

#73517

Purpose

TagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-T

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::TagRFP-T::PH::tbb-2

Use

Tags

TagRFP-T

Expression

Mutation

Promoter

mes-5

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-SIV-RCM-vpx

Plasmid

#115844

Purpose

Encodes codon optimized FLAG tagged SIVRCM-Vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVRCM-Vpx

Use

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-CH111gp160

Plasmid

#123268

Purpose

Mammalian expression plasmid for Env from the CH111 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (AD8) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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