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Plasmid#111858PurposeN-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1 (Bicdl1 Mouse)
UseTagsHis-tag, SNAPf-tag, TEV site, and ZZExpressionInsectMutationR250Q, Optimised for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDTFendoNA2
Plasmid#174731PurposeExpresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.DepositorInsertDT386F
UseTagsExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES
Plasmid#182485PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-(HA)3-ubiquitin
Plasmid#196991PurposeExpresses HA-tagged ubiquitin in mammalian cellsDepositorInsertubiquitin (UBC Human)
UseTagstriple HA tagExpressionMammalianMutationsilent mutation in ubiquitin codon 13PromoterCMVAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-NLS-BirA-2A-mCherry_Ras
Plasmid#80067PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
tetO-ESRRG
Plasmid#170684Purposedoxycycline-inducible overexpression of ESRRGDepositorInsertESRRG (ESRRG Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDR1-GFP
Plasmid#111657PurposeC-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1-GFP (Bicdl1 Mouse)
UseTags8xHis-tag, GFP, TEV site, and ZZExpressionInsectMutationOptimised for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 EWS-myc-HIS
Plasmid#46386Purposeexpresses myc, HIS tagged EWSR1 in mammalian cellsDepositorInsertEWSR1 (EWSR1 Human)
UseTagsHis and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-cytoBirA-2A-mCherry_Ras
Plasmid#80066PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX neo ACTN1-GFP, Y246E
Plasmid#138294PurposeMammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP, Y246E fusion proteinDepositorInsertACTN1 (ACTN1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationY246EPromoterCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDN(1-400)-GFP
Plasmid#111862PurposeC-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDN(1-400)-GFP (Bicd2 Mouse)
UseTags8xHis tag, GFP, and ZZ tagExpressionInsectMutationCodon optimized for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT-EPHX1
Plasmid#53114PurposeIn vitro translation of microsomal epoxide hydrolaseDepositorInsertEPHX1 (EPHX1 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterCMV and T7Available sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Hook3(1-522)-SNAPf-Psc-StrepII
Plasmid#111860PurposeC-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.DepositorInsertHook3(1-552)-SNAPf (HOOK3 Human)
UseTagsPsc, SNAPf, and Strep-IIExpressionInsectMutationOptimised for expression in Sf9 cellsPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-B-838;+80pCalb2
Plasmid#66750Purposeluciferase reporter for Calb2 promoter (-838 to +80, WT)DepositorInsertCalb2 promoter (-838bp +80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cntn6-AP-His
Plasmid#71944PurposeExpresses the extracellular region of the Contactin 6 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertCntn6 (Cntn6 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hook3(1-522)-GFP-Psc-StrepII
Plasmid#111861PurposeC-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.DepositorInsertHook3(1-552)-GFP (HOOK3 Human)
UseTagsGFP, Psc tag, and Strep tagExpressionInsectMutationOptimised for expression in SF9 cellsPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
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tetO-IRX5
Plasmid#170693Purposedoxycycline-inducible overexpression of IRX5DepositorInsertIRX5 (IRX5 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-ZFPM2
Plasmid#170707Purposedoxycycline-inducible overexpression of ZFPM2DepositorInsertZFPM2 (ZFPM2 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutation3 nonsynonymous changes: 353C>T (Ser>Phe)…PromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-ubiquitin
Plasmid#196999PurposeExpression of GAL4 DNA-binding domain (BD)-ubiquitin fusion protein in yeast (yeast two-hybrid assays)DepositorInsertubiquitin (UBC Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only