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Plasmid#227096PurposeLuciferase reporter vector with wild type 5' Element (MMTV) insertDepositorInsertCMV Promoter and R to 400bp Gag
UseLuciferaseTagsExpressionMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMamCol1
Plasmid#215184PurposeAllows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coliDepositorInserttsPurple
UseAAV, CRISPR, and Synthetic Biology ; Recombinant …TagsSNAC-StrepII tagExpressionBacterial and MammalianMutationPromoterTetON 3G Bidirectional for Mammalian and Trc with…Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK24
Plasmid#210597PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdACTINpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdACTINproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-TdIF2
Plasmid#196906Purposevisualization of TdIF2/ERBP as a fusion to mCherryDepositorInsertTdIF2
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TdIF2
Plasmid#199699Purposevisualization of nucleolus in living mammalian cellsDepositorInsertTdIF2
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES (M)
Plasmid#182437PurposeYeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS(M)
NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-WT-eGFP-KDEL
Plasmid#177727PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminusDepositorInserthFluc
UseTagsKDEL, Prolactin Signal Sequence, and eGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-WT-KDEL
Plasmid#177715PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retentionDepositorInserthFluc
UseTagsKDEL and Prolactin Signal SequenceExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis
Plasmid#176575PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184Q W330YPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis
Plasmid#175788PurposePlasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184QPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pV5endoNA2
Plasmid#174729PurposeExpresses V5-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertV5 epitope tag
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJS263
Plasmid#134141PurposeBacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)DepositorInsertSka1 MTBD-His (Y106G6H.15 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW087
Plasmid#134128PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
UseTagsGFPExpressionBacterialMutationK183A K184APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW084
Plasmid#134130PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
UseTagsGFPExpressionBacterialMutationR236APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW095
Plasmid#134131PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
UseTagsGFPExpressionBacterialMutationR155APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW090
Plasmid#134129PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
UseTagsGFPExpressionBacterialMutationK223A K226APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW093
Plasmid#134137PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, R155APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW082
Plasmid#134136PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, R236APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only