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pCMV_SEPT9_i1 DN7-msfGFP

Plasmid

#180335

Purpose

mammalian expression of human SEPT9_i1 DN7 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 DN7

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 Gmut-msfGFP

Plasmid

#180331

Purpose

mammalian expression of human SEPT9_i1 Gmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 W520A, H530D

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 NCmut2-msfGFP

Plasmid

#180330

Purpose

mammalian expression of human SEPT9_i1 NCmut2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 I281D, M288D

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-H2b-Che

Plasmid

#179650

Purpose

mCherry co-transfection marker

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-HRAS-G12V

Plasmid

#179652

Purpose

HRAS active mutant (G12V mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

HRAS (HRAS Human)

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-P3-cTVMVP

Plasmid

#179664

Purpose

ER-associated uncaged-cTVMVP with P3 leucine zipper

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

cTVMVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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AiP906-pCMV-oBxb1

Plasmid

#181777

Purpose

Bxb1-in vector

Depositor

Tanya Daigle

Insert

oBxb1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iDianiSnFR_PM

Plasmid

#177751

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iDianiSnFR_PM

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iDianiSnFR_ER

Plasmid

#177750

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iDianiSnFR_ER

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iCyt_BrEt_SnFR_PM

Plasmid

#177747

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iCyt_BrEt_SnFR_PM

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
_pCMV(MinDis)-iCyt_F_SnFR_PM

Plasmid

#177745

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

_pCMV(MinDis)-iCyt_F_SnFR_PM

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iCytSnFR_PM

Plasmid

#177743

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iCytSnFR_PM

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iCyt_BrEt_SnFR_ER

Plasmid

#177746

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iCyt_BrEt_SnFR_ER

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
_pCMV(MinDis)-iCyt_F_SnFR_ER

Plasmid

#177744

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

_pCMV(MinDis)-iCyt_F_SnFR_ER

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iCytSnFR_ER

Plasmid

#177742

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iCytSnFR_ER

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-PGRMC1-21-195

Plasmid

#173473

Purpose

PGRMC1 lacking the N-terminal region upstream of the transmembrane region

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deleted for amino acids 1-20

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-luc-miR-1

Plasmid

#171556

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-1

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-null (BPK1077)

Plasmid

#171128

Purpose

Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

pCMV and T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A T31A-NG-nCas9

Plasmid

#163539

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A T31A-NG-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

Promoter

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A T31A-nCas9

Plasmid

#163538

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A T31A-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)eA3A T31A

Promoter

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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