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We narrowed to 4,144 results for: Pet2;

Showing: 3451 - 3480 of 4144 results

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SARS-CoV-2 Mpro protease (M165I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204778

Purpose

Bacterial expression of Sars Cov 2 MPRO M165I mutant

Depositor

Frank von Delft, ASAP Discovery

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

M165I

Promoter

Available since

Dec. 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-like

Plasmid

#204769

Purpose

Bacterial expression of Sars Cov 2 MPRO C145S mutant

Depositor

Frank von Delft, ASAP Discovery

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

C145S mutant

Promoter

Available since

Nov. 30, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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H6_fold-Z _Gogy

Plasmid

#201828

Purpose

Expresses H6_fold-Z _Gogy in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H6_fold-Z _Gogy

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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H7_fold-K _Mussoc

Plasmid

#201830

Purpose

Expresses H7_fold-K _Mussoc in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H7_fold-K _Mussoc

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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H6_fold-U _Nomur

Plasmid

#201829

Purpose

Expresses H6_fold-U _Nomur in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H6_fold-U _Nomur

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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H6_fold-C _Rei

Plasmid

#201827

Purpose

Expresses H6_fold-C _Rei in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H6_fold-C _Rei

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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H5_fold-0 _Chantal

Plasmid

#201826

Purpose

Expresses H5_fold-0_Chantal in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H5_fold-0 _Chantal

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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FnR8-10

Plasmid

#182569

Purpose

Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 8-10

Use

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1379A

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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His8OphMA-21mer.

Plasmid

#189670

Purpose

His8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.

Depositor

James Naismith, Markus Künzler

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMATEV18mer

Use

Tags

His8 tagged

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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His8OphMADeltaC6-18mer.

Plasmid

#189671

Purpose

His8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptides

Depositor

James Naismith, Markus Künzler

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMA∆C6-TEV18mer

Use

Tags

His8 tagged

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Xalb_I-F1_array_GG

Plasmid

#178770

Purpose

Golden Gate vector to generate X. albilineans type I-F1 single arrays

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

Golden Gate vector BaeI recognition site to generate X. albilineans type I-F1 single arrays

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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Xalb_I-C_array_GG

Plasmid

#178767

Purpose

Golden Gate vector to generate X. albilineans type I-C single arrays

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

Golden Gate vector BaeI recognition site to generate X. albilineans type I-C single arrays

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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C9Y9E7

Plasmid

#163230

Purpose

Inducible expression of UniProt identifier C9Y9E7

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

C9Y9E7

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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E6SCX5

Plasmid

#163275

Purpose

Inducible expression of UniProt identifier E6SCX5

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

E6SCX5

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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F0TBY7

Plasmid

#163245

Purpose

Inducible expression of UniProt identifier F0TBY7

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

F0TBY7

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas5

Plasmid

#170090

Purpose

expresses E. coli type I-E Cas5

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas5

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas7

Plasmid

#170092

Purpose

expresses E. coli type I-E Cas7

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas7

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEcCas8

Plasmid

#170093

Purpose

expresses E. coli type I-E Cas8

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas8

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCse2

Plasmid

#170094

Purpose

expresses E. coli type I-E Cse2

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cse2

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJH767

Plasmid

#172172

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WT]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB]

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH857

Plasmid

#172175

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WTopt]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDH010

Plasmid

#157645

Purpose

sMHT-113 fused non-dimerizing GRX2-C37A mutant

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-GRX2_C37A
GRX2_C37A-sMHT(114-230)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2004

Plasmid

#139889

Purpose

Expression plasmid for LetAB with 6 ring (rings 123457) LetB (without TM region) mutant

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta634-746)-6xHis

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1993

Plasmid

#139897

Purpose

Expression plasmid for LetB PLL1 deletion (without TM region)

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta114-128, P113G)-6xHis

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1999

Plasmid

#139903

Purpose

Expression plasmid for LetB PLL7 deletion (without TM region)

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta816-826, S815G, T827G)-6xHis

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2080

Plasmid

#155160

Purpose

Expresses Strep-MlaF_L266A)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF_L266A

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2081

Plasmid

#155161

Purpose

Expresses (Strep-MlaF_Y261A_L265A_L266A)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF(Y261A_L265A_L266A)

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1305

Plasmid

#155143

Purpose

Expresses MlaFB, His tag on MlaF N-term

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(6xHis-TEV-MlaF)-MlaB

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1306

Plasmid

#155144

Purpose

Expresses MlaFB, His tag on MlaF C-term

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(MlaF-6xHis)-MlaB

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1307

Plasmid

#155145

Purpose

Expresses MlaFB, His tag on MlaB N-term

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge