-
Plasmid#206041PurposeHaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTagDepositorTypeEmpty backboneUseTagsHaloTag and SpyCatcher-8HisExpressionBacterialMutationPromoterT7Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only
-
pLX313 MSLN
Plasmid#193617PurposeExpresses MSLNDepositorInsertMSLN (MSLN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dual-sgRNA_hU6-mU6
Plasmid#154194PurposeLentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lck-mTurquoise2
Plasmid#98822PurposeIn vivo visualization of the plasma membrane (can be used for colocalization studies)DepositorArticleInsertLck
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-pBAD
Plasmid#108315PurposeGFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.DepositorInsertGFP under control of arabinose-inducible promoter pBAD
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
hCD4-mOrange
Plasmid#110192PurposeEncodes for human CD4 coding sequence tagged with the mOrange FPDepositorInserthCD4 (CD4 Human)
UseTagsmOrangeExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3e UbB polyA
Plasmid#188702PurposeGateway 3' element encoding the ubiquitin B polyadenylation sequenceDepositorInsertUbB polyA (ubb Zebrafish)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mCherry-C
Plasmid#203326PurposeExpress N-terminal mCherry-fused protein in mammalian cellDepositorTypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
N-Cadherin-EGFP
Plasmid#18870DepositorInsertN-Cadherin (Cdh2 Mouse)
UseTagsEGFP, Kanamycin, and N-CadherinExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
TA1-Tango
Plasmid#66508PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertTA1 (TAAR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV PIG (Puro IRES GFP empty vector)
Plasmid#21654PurposeRetroviral backbone for cloning and gene expression. Select with puromycin or GFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsGFP and IRESExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-miRFP670nano3
Plasmid#184664PurposeMonomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmidDepositorInsertmiRFP670nano3
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-Halotag-FRB-EX-nes_pLX304
Plasmid#120913PurposeLentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosolDepositorInsertHA-Halotag-FRB-EX-NES
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCherryGFP-CoV2-Inframe-stop
Plasmid#177620PurposeDual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).DepositorInsertSARS-CoV-2 FSE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCherryGFP-Inframe-noFSE
Plasmid#177619PurposeDual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).DepositorInsertNone
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-KDR
Plasmid#23925DepositorInsertKDR (KDR Human)
UseGateway donor vectorTagsExpressionMutationV297IPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
G5-CD40
Plasmid#121166PurposepMP71 vector expressing GFP - P2A - G5 - myc - CD40DepositorInsertG5-CD40
UseRetroviralTagsGFP and mycExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
ABE8r-R153del
Plasmid#208296PurposeExpresses ABE8r in mammalian cellsDepositorInsertTadA8r-R153del-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-GFP
Plasmid#11154PurposeMammalian expression vector for expression of GFP (EF1a promoter)DepositorUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-H1.5
Plasmid#32898DepositorInsertHistone H1.5 (H1-5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHREAC
Plasmid#134908PurposeOverexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterdouble 35SAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG364-greenFAST
Plasmid#160353Purposeexpresses greenFAST in mammalian cellsDepositorInsertgreenFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK
Plasmid#79120PurposeLentiviral vector for constitutive expression of genes (PGK promoter)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC_mClover3_BSDminus_F0
Plasmid#184165PurposeDonor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmClover3
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG365-redFAST
Plasmid#160354PurposeExpresses redFAST in mammalian cellsDepositorInsertredFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP
Plasmid#137763PurposeControl plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.DepositorInsertGFP
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-5HRE-GFP PuroR
Plasmid#128958PurposeLentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent proteinDepositorInsert5X HRE of VEGF (VEGFA Human)
UseLentiviralTagsd2EGFPExpressionMammalianMutationPromoterminimal CMV promoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPTN-EGFP
Plasmid#27052DepositorInsertoptineurin (OPTN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-NFIA
Plasmid#141403PurposeInducible Expression of NFIADepositorInsertNFIA (NFIA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC2A1
Plasmid#132103PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC2A1 (SLC2A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits