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Plasmid#113687PurposeSaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.DepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ABE8.8-d
Plasmid#136295Purposeexpresses ABE8.8-d in mammalian cellsDepositorInsertABE8.8-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)
Plasmid#36881DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp2-a C2AB
Plasmid#40051DepositorInserthSlp2-a C2AB (SYTL2 Human)
UseTagsEGFPExpressionMammalianMutationC2AB domains only (aa 597-910)PromoterCMV promoterAvailable sinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Donor/Reporter-COL4A5
Plasmid#130280PurposeCOL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor templateDepositorInsertmCherry-eGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp2-a del.SHD
Plasmid#40050DepositorInserthSlp2-a del.SHD (SYTL2 Human)
UseTagsEGFPExpressionMammalianMutationdeletion of SHD domain (1-56)PromoterCMV promoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a del.SHD
Plasmid#40036DepositorInserthSlp4-a del.SHD (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationdeleted SHD domain (1-143)PromoterCMV promoterAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSc1
Plasmid#80436PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6Available sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAC-CLEC4D
Plasmid#172411PurposeMAC-tagged gene expressionDepositorInsertC-type lectin domain family 4 member D (CD antigen CD368) (CLEC4D Human)
UseTagsMAC-tagExpressionMammalianMutationPromoterCMV promoterAvailable sinceNov. 4, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSc2
Plasmid#80437PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a C2AB
Plasmid#40037DepositorInserthSlp4-a C2AB (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationC2A and C2B domains (353-671)PromoterCMV promoterAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a C2A
Plasmid#40038DepositorInserthSlp4-a C2A (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationC2A domain (353-487)PromoterCMV promoterAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a C2B
Plasmid#40039DepositorInserthSlp4-a C2B (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationC2B domain (488-671)PromoterCMV promoterAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
ABE8.13-d
Plasmid#136297Purposeexpresses ABE8.13-d in mammalian cellsDepositorInsertABE8.13-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp2-a V18A
Plasmid#40054DepositorInserthSlp2-a V18A (SYTL2 Human)
UseTagsEGFPExpressionMammalianMutationValine 18 to AlaninePromoterCMV promoterAvailable sinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Slp2-a C2B
Plasmid#40053DepositorInsertSlp2-a C2B (Sytl2 Mouse)
UseTagsEGFPExpressionMammalianMutationC2B domainPromoterCMV promoterAvailable sinceNov. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-TACC3KDP∆682-688-shTACC3
Plasmid#69116PurposeDual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationDeletion of amino acids 682-688 (region containin…PromoterCMVAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Slp2-a E11A/R32A
Plasmid#40055DepositorInsertSlp2-a E11A/R32A (Sytl2 Mouse)
UseTagsEGFPExpressionMammalianMutationGlutamate 11 to Alanine, Arginine 32 to AlaninePromoterCMV promoterAvailable sinceNov. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Slp2-a C2A
Plasmid#40052DepositorInsertSlp2-a C2A (Sytl2 Mouse)
UseTagsEGFPExpressionMammalianMutationC2A domainsPromoterCMV promoterAvailable sinceNov. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a K>Q
Plasmid#40044DepositorInserthSlp4-a K>Q (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationLysine 410,412,416,564,566,571, 572 to GlutaminePromoterCMV promoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only