-
Plasmid#60673Purposeoverexpression of M. tuberculosis ddl gene in MycobacteriumDepositorInsertddl
UseE.coli-mycobacterium shuttle vectorTags6 codons of M. bovis BCG hsp60ExpressionMutationPromoterBCG Hsp60Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site
Plasmid#228633PurposeBacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)DepositorInsertEnterovirus Coxsackievirus A71 2A protease
UseTagsHis-SUMOExpressionBacterialMutationC110APromoterAvailable sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJB72
Plasmid#226312PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1
UseTagsExpressionBacterialMutationY300APromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB44
Plasmid#226303PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S MR1
UseTagsExpressionBacterialMutationC292S, C310SPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB43
Plasmid#226302PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertWT CsoS2, frameshift site intact
UseTagsExpressionBacterialMutationPromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM1
Plasmid#225288PurposeE. coli- C. coli shuttle vector; KanRDepositorInsertReplication elements from pWestmill 11
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 msNLRP3 (STOP)
Plasmid#218638PurposeGateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ301
Plasmid#205415PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Hs1Cas12a without promoterDepositorInsertHs1Cas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSM-dCas9::Ap
Plasmid#154307PurposeShuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Cka2
Plasmid#99980PurposeShuttle yeast Cka2 into Gateway destination vectorDepositorInsertCka2 (CKA2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Utp16
Plasmid#99985PurposeShuttle yeast Utp16 into Gateway destination vectorDepositorInsertUtp16 (BUD21 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pESC-leu2d(empty)
Plasmid#20120DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pST-KT
Plasmid#44561Purposefor ectopic inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterUV15 promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pST-K
Plasmid#44560Purposefor ectopic constitutive expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterUV15 promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptac-sfGFP
Plasmid#139934PurposeIPTG-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertLacI-Ptac-sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYHY3
Plasmid#216109PurposeaTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.DepositorInsertNanoLuc
UseSynthetic Biology; E. coli-bacteroides shuttle ve…Tags3xFLAG tag and BT3630 signal peptideExpressionBacterialMutationPromoterP1TDP-A21Available sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265_OoA3GX2
Plasmid#225147PurposeGateway entry clone (attL1 & attR5) OoA3GX2-CBE_V01 for C-T base editingDepositorInsertOoA3GX2-zCas9(D10A)-UGI
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_PflaB-mCherryBb
Plasmid#118243PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherryDepositorInsertflagellin promoter driven mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_P0826-mCherryBb
Plasmid#118242PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0826 promoter driven mCherryDepositorInsertbb0826 promoter driven mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQP-iRIS
Plasmid#102584PurposeRegulation of protein localization (near-infrared-blue-light-inducible shuttle)DepositorInsertBphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only