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Plasmid#161956PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsGFPuv His8ExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only
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pCGFP-BC
Plasmid#161959PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsGFPuv His8ExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pNGFP-EU
Plasmid#161967PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsEGFP His8ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCGFP-EU
Plasmid#161968PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsEGFP His8ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
PG13-mCherry
Plasmid#195291PurposemCherry reporter containing 13 copies of the p53-binding consensus sequenceDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur
Plasmid#60507PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-RP
Plasmid#60497PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-GP
Plasmid#60511PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RP
Plasmid#60513PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-GB
Plasmid#60520PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla
Plasmid#60510PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-GH
Plasmid#60498PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-BH
Plasmid#60499PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPSU20-40-60-80
Plasmid#173839PurposeCoexpresses 20, 40, 60 and 80 kD Penn State ladder proteinsDepositorInsertSTRHSTPABPACCBPv3
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-RB
Plasmid#60506PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RB
Plasmid#60522PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-GP
Plasmid#60495PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance geneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
EJ2GFP-puro
Plasmid#44025DepositorInsertEJ2GFP egfp-based chromosomal break reporter
UseTagsExpressionMammalianMutationinsertion of I-SceI, 3 frame stop, and flanking m…PromoterpCAGGSAvailable sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
ER_K20
Plasmid#133861PurposeExpression of ER-targeted Ribosome stalling reporterDepositorInsertER_K20
UseTagsEGFP and RFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cyto_K20
Plasmid#133862PurposeExpression of cytoplasmic Ribosome stalling reporterDepositorInsertCyto_K20
UseTagsEGFP and RFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only