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Plasmid#113832Purposetargeting osTIR1-GFP into mouse Actb locus for auxin inducible degradationDepositorInsertosTIR1-GFP (NEWENTRY Synthetic)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Halo-Erk2 donor
Plasmid#113104PurposeDonor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequenceDepositorInsertN terminal Erk2 donor region including homology arms and HaloTag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-siRNA control
Plasmid#10900DepositorInsertsiGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_human_spike-in
Plasmid#125152PurposeEmpty vector to clone and measure the expression of promoter candidates (spike-in) by determining the abundance of transcripts originating from the candidate in human cellsDepositorTypeEmpty backboneUseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-2 SNAP-Syk tSH2
Plasmid#113028Purposefor purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.DepositorInsertSNAP-Syk tSH2
UseTagsSNAPExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP N1 Ub-CRT
Plasmid#69619PurposeExpress cytoplasmic mature CRT.DepositorInsertUbiquitina-Calreticulin (mature)
UseTagsEGFP and UbiquitinExpressionMammalianMutationDeletion of signal peptide of calreticulin at the…PromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B7-deltaE4
Plasmid#122560PurposeModified block 7, with deletion in E4DepositorInsertAdenovirus 5 genomic region 30888-35938
UseSynthetic BiologyTagsExpressionMutationAdenovirus sequences 32874-35461 replaced with Sp…PromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-REP
Plasmid#64966Purposemini-Tn7 vector with oriR6K, for attTn7 site determinationDepositorInsertoriR6K
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGKO2-Gateway
Plasmid#63618Purposea binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants. Uses Gateway cloning.DepositorTypeEmpty backboneUseFungal expressionTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-sfGFP
Plasmid#180757PurposepET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH462
Plasmid#172154PurposepylBCD variant in pBAD backboneDepositorInsertPara (Sumo-tagged pylBCD); AraC
UseTagsExpressionBacterialMutationSUMO tag fused to N-terminus of PylBPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Hbxadr-Hatag
Plasmid#24930DepositorInsertHBx
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLIA-E p27 (CC#620)
Plasmid#15191DepositorInsertp27 (Cdkn1b Mouse)
UseRetroviralTagsFlag and HisExpressionMammalianMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPEE17
Plasmid#128361PurposeDetecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.DepositorInsertAcetamidase (amdS)
UseUnspecifiedTagsExpressionMutationPromoterTEF1Available sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3H
Plasmid#110080PurposeAnalogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM
Plasmid#183077PurposePlasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.DepositorInsertCas12c tracrRNA and crRNA ending with a HDV ribozyme
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCW01
Plasmid#72300PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19TagsExpressionBacterialMutationPromoterlacAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKDH
Plasmid#67584PurposeGene expression in plants. A promoter and/or reporter can be cloned into this plasmidDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNoneAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJ111
Plasmid#78707PurposeModular vector for constitutive secreted amylase reporter expressionDepositorInsertamyEopt
UseTagsExpressionBacterialMutationPromoterPvegAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only