Addgene

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pKSCTMF

Plasmid

#85073

Purpose

Gene insertion/deletion in Pichia pastoris

Depositor

Sheng Yang

Article

Yang et al FEMS Yeast Res. 2009 Jun;9(4):600-9. doi

Insert

AOX1promoter - mazF - AOX1 tt- Zeocin R gene

Use

Gene insertion/deletion in pichia pastoris

Tags

Expression

Mutation

Promoter

pAOX1

Available since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A-1x5

Plasmid

#58769

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mCit-IRES-Neo

Plasmid

#155230

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mCitrine sensor

Use

Lentiviral

Tags

mCitrine

Expression

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Foxa2

Plasmid

#33004

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A2 (Foxa2 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only
pGEX TIF1gamma

Plasmid

#15734

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma (TRIM33 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
N-GFP-Dcp2

Plasmid

#163585

Purpose

express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

DCP2 promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJH291

Plasmid

#172153

Purpose

Reporter plasmid for pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para sfGFP.2TAG; Plpp pylS; PproK pylT; AraC

Use

Tags

Expression

Bacterial

Mutation

sfGFP(N39*, Y151*)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-GFP Smad4

Plasmid

#15724

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad4 shRNA (SMAD4 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
mCherry-Sec61β

Plasmid

#172445

Purpose

Expression of ER protein Sec61beta with an N-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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MO70 Orai1 N223A

Plasmid

#21663

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai1 (ORAI1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

N223A

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
TRPML2-YFP

Plasmid

#18830

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-HBH-kanMX6

Plasmid

#26872

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag

Use

Pcr tagging yeast

Tags

Expression

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pX330A-1x7

Plasmid

#58771

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pHACK-GAL4>QF2(newHA1)

Plasmid

#104873

Purpose

HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

T2A-QF2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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Venus-KRasCT

Plasmid

#99016

Purpose

Membrane marker

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

Venus KRasCT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CM

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mE6-AP/2591a

Plasmid

#11468

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

mE6-AP (Ube3a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

n/a

Promoter

Available since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
mCherry-KRasCT

Plasmid

#99017

Purpose

Membrane marker

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

mCherry KRas CT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG1

Plasmid

#193799

Purpose

Protein production of ChemoG1 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG1

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pKOdeltagalK (Kan)

Plasmid

#110089

Purpose

Analogous to pKO but without the negative selection gene galK.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Tags

Expression

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO MycLAP-STIL

Plasmid

#80266

Purpose

mammalian expression plasmid for MycLAP-STIL

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Use

Tags

Myc-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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