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Plasmid#50990PurposeExpresses RALB in mammalian cellsDepositorInsertRALB (RALB Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
gCOTS-pyl
Plasmid#92048PurposeThis is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.DepositorInsertsPyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseCyanobacteria s. elongatus genome recombination i…TagsExpressionMutationPromoterLeuP (native S. elongatus promoter) and PrcbLAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-MEKK1-1-1493
Plasmid#21632DepositorInsertmitogen-activated protein kinase kinase kinase 1 (Map3k1 Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-1 Jip4 (1-272)
Plasmid#59002PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsGstExpressionBacterialMutationJip4 (aa1-272)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-272)
Plasmid#58928PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-272aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-151)
Plasmid#58926PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-151aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-212)
Plasmid#58927PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-212aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX RalB G23V ΔC
Plasmid#50995PurposeExpresses RalB G23V ΔC in bacterial cellsDepositorInsertRALB (RALB Human)
UseTagsGSTExpressionBacterialMutationG23V ΔCPromotertac_promoterAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLTR PKC gamma
Plasmid#8418DepositorInsertProtein Kinase C gamma (Prkcg Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC12
Plasmid#205068PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 12 amino acidsPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC16
Plasmid#205069PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 16 amino acidsPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC18
Plasmid#205070PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 18 amino acidsPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC20
Plasmid#205071PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 20 amino acidsPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
mAMCase_CatD_6xHis_pTwistCMV
Plasmid#200228PurposeExpresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vectorDepositorInsertAcidic Mammalian Chitinase (Chia1 Mouse)
UseTags6xHisExpressionMammalianMutationS354FPromoterCMVAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
mAMCase_CatD_6xHis_pcDNA3.1
Plasmid#200229PurposeExpresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pcDNA 3.1 vectorDepositorInsertAcidic Mammalian Chitinase (Chia1 Mouse)
UseTags6xHisExpressionMammalianMutationS354FPromoterCMVAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC9
Plasmid#205067PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 9 amino acidsPromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/sh-FYN1-GFP_Seq_C
Plasmid#201399PurposeKnockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertFYN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPL13-ECFP-SNAP
Plasmid#187967Purposeexpresses RPL13 with C-terminal tags ECFP and SNAPDepositorInsertRPL13 (RPL13 Human)
UseTagsECFP-SNAPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r
Plasmid#162965PurposeGateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expressionDepositorInsertHis6-Halotag fusion tag with upstream E. coli ribosome binding site
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r
Plasmid#162964PurposeGateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expressionDepositorInsertHis6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only