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pLacIBD::SDRX (GB0673)

Plasmid

#68183

Purpose

Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

LacI Binding Domain fused to SRDX Transcriptional Repressor

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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PatoL-OG241

Plasmid

#72841

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoL

Use

Tags

Expression

Bacterial

Mutation

Promoter

PatoL

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pI pm

Plasmid

#218098

Purpose

Phagemid that expresses LacI, and pI under the Lac promoter

Depositor

Kevin Esvelt

Insert

pI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pII pm

Plasmid

#218099

Purpose

Phagemid that expresses LacI, and pII under the Lac promoter

Depositor

Kevin Esvelt

Insert

pII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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DT049A

Plasmid

#159410

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli tet promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tet-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

tet promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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DT033A

Plasmid

#159394

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli tac promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tac-BDNF (Bdnf, Mouse) (Bdnf Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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PatoL-LOG241

Plasmid

#72846

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoL

Use

Tags

Expression

Bacterial

Mutation

Promoter

PatoL

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMGF172

Plasmid

#96998

Purpose

ish1 mCherry insertion cassette with ura4+ gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Use

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + ura4(…

Promoter

N/A

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLexA::SRDX (GB0732)

Plasmid

#68184

Purpose

Provides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

LexA Binding Domain fused to SRDX Transcriptional Repressor

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGal4BD::BDR (GB0734)

Plasmid

#68185

Purpose

Provides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Gal4 Binding Domain fused to BDR Transcriptional Repressor

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBOB-EF1-FastFUCCI-Puro

Plasmid

#86849

Purpose

3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

Depositor

Duncan Jodrell, Kevin Brindle

Article

Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

Inserts

mKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1 and PGK

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPR-HOT-P2A-Clover-BlastR

Plasmid

#138568

Purpose

Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

Depositor

Hans Clevers

Article

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

P2A-clover-BlastR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJC5-4-insulator-BREreporter-insulator

Plasmid

#171499

Purpose

injections to make Bmp reporter mouse transgenic line

Depositor

James Briscoe

Article

Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

Insert

Insulator BRE-MLP:H2B-Turqoise

Use

Tags

Expression

Mammalian

Mutation

Promoter

BRE-MLP

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only
pUPD P35S (GB0552)

Plasmid

#160554

Purpose

35s promoter for N-terminal fusions

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Nucleic Acids Res. 2017 Feb 28;45(4):219

Insert

35s

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Ascl1

Plasmid

#74338

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Ascl1 (also known as Mash1). Not for gene expression.

Depositor

Peter Mombaerts

Article

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

achaete-scute family bHLH transcription factor 1 (Ascl1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMGF157

Plasmid

#96972

Purpose

ish1-mCherry insertion cassette with Hygro resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Use

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + hygro…

Promoter

N/A

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMGF170

Plasmid

#96997

Purpose

ish1-mCherry insertion cassette with NAT resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Use

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + NAT r…

Promoter

N/A

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

Plasmid

#221447

Purpose

Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

SPR degron: hGEM (1-110) fragment that is fused t…

Promoter

CMV

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMGF130

Plasmid

#96971

Purpose

S. pombe lem2 knockout cassette with hygro resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

lem2 (lem2 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

whole ORF deletion and replaced with hygro resist…

Promoter

N/A

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMGF169

Plasmid

#96996

Purpose

ish1 mCherry insertion cassette with G418 resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Use

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + G418 …

Promoter

N/A

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge