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Plasmid#206864PurposeTo stably express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag in cells resistant to Neo.DepositorInsertKras (Kras Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationchanged Glutamine 61 to ArgininePromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mGCC2-FLAG
Plasmid#214166PurposeTransient expression of GCC2DepositorInsertGCC2 (Gcc2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-K142R
Plasmid#213423PurposeExpresses human SKP1 K142R mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-K142R (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBOBI N-FLAG HIPK2
Plasmid#213004Purposeexpresses mouse HIPK2DepositorInsertHIPK2 (Hipk2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-T131A
Plasmid#213482PurposeExpresses human SKP1 T131A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-97-101-5A
Plasmid#213642PurposeExpresses human SKP1-97-101-5A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1--97-101-5A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-K163R
Plasmid#213424PurposeExpresses human SKP1 K163R mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-K163R (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-T131E
Plasmid#213601PurposeExpresses human SKP1 T131E mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131E (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA
Plasmid#191528PurposeVector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabainDepositorInsertSLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via hdr using ouabainTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
IAA17-4flag-HIS5
Plasmid#207015PurposeFor ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 flag-cFLIP
Plasmid#211529PurposeExpresses flag-tagged cFLIP (CFLAR)DepositorInsertcFLIP (CFLAR Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-C17orf53-FLAG
Plasmid#211447Purposegeneration of Bacmid for insect cell expression of C17orf53 C-terminally fused to FLAG-TagDepositorInsertC17orf53 (HROB Human)
UseTagsFLAG-TagExpressionInsectMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pDEST-3xFLAG-Ubc7
Plasmid#212801PurposeConstitutive expression of 3xFLAG-tagged budding yeast Ubc7 in mammalian cellsDepositorInsertUbc7
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24-1~93
Plasmid#208633Purposefor E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93DepositorInserthuman RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationonly aa 1~93, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
MIGR1_FLAG_TAL1-long_dt tomato
Plasmid#210639PurposeOverexpression of TAL1-longDepositorInsertTAL1-long (TAL1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-FLAG-HA-mmuMettl3
Plasmid#207122PurposeLentiviral plasmid to express mouse Mettl3DepositorInsertMettl3 (Mettl3 Mouse)
UseLentiviralTagsFLAG-HAExpressionMutationPromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-FLAG-HA-mmuMettl3-LN
Plasmid#207129PurposeLentiviral plasmid to express mouse Mettl3 Lambda peptide C-terDepositorInsertMettl3 (Mettl3 Mouse)
UseLentiviralTagsFLAG-HA and Lambda Peptide (LN)ExpressionMutationPromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-RPT6-FLAG
Plasmid#205416PurposeExpresses dCas9-RPT6 with FLAG labelDepositorInsertdCas9-RPT6 (Psmc5 Rat)
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Mts
Plasmid#195065PurposeExpresses FLAG-tagged Drosophila mts proteinDepositorInsertmicrotubule star (mts Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only