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Plasmid#186034PurposeExpress VHH F8-2 as a mCherry fusion in mammalian cellsDepositorInsertVHH F8-2
UseTagsmCherryExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TRIM28
Plasmid#45568DepositorInsertTRIM28 (TRIM28 Human)
UseTagsEGFPExpressionMammalianMutationG34S mutation in EGFPPromoterCMVAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-EFNB2-RBD-Fc (WT)
Plasmid#200978PurposeMammalian expression plasmid for soluble EFNB2 (wild type) fused to IgG1 FcDepositorInsertEFNB2 (EFNB2 Human)
UseTagsIgG1 FcExpressionMammalianMutationSoluble Receptor-Binding DomainPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKH3-TRIM28
Plasmid#45569DepositorInsertTRIM28 (TRIM28 Human)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
Fuw-dCas9-Dnmt3a-P2A-tagBFP
Plasmid#84569PurposeLentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFPDepositorInsertdCas9-Dnmt3a (DNMT3A Human, Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
Plasmid#35512PurposeAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsmCherryExpressionMammalianMutationE123T and T159CPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
PXPR007 sgCIC-2
Plasmid#74959PurposeCas9 + sgCIC-2 with blasticidin selectionDepositorInsertCIC (CIC Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT_KCNQ3
Plasmid#173163PurposeTo generate Flp-In CHO-KCNQ3 stable cellsDepositorInsertKCNQ3 (KCNQ3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-HA
Plasmid#61355Purposeencodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal HA tag
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3 GCN1 R2312A-3xFlag_IRES-iRFP
Plasmid#198389PurposeGCN1 R2312A expressonDepositorInsertGCN1 (GCN1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationR2312A mutation in RWDBD (RWD binding domain)PromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PXPR007 sgCIC-1
Plasmid#74953PurposeCas9 + sgCIC-1 with blasticidin selectionDepositorInsertCIC (CIC Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
BglB in pET29b(+)
Plasmid#55773PurposeExpresses beta-glucosidaseDepositorInsertBeta-glucosidase B (bglB Synthetic)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP
Plasmid#155306PurposeRfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFPDepositorInsertRfx Cas13d CRISPR RNA BFP
UseTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IKZF1-V2-V5
Plasmid#107390PurposeMammalian expression of IKZF1-V2-V5DepositorInsertIKZF1-V2 (IKZF1 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY30
Plasmid#84745PurposeExpresses huAsCpf1-P2A-puro and crRNA guideDepositorInsertshuAsCpf1
puromycin resistance gene
UseTags3xHA, NLS, and T2AExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRB_Hormone-recep
Plasmid#109909PurposeProtein expression and purification of ESRRB_Hormone-recepDepositorInsertESRRB_Hormone-recep (ESRRB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-AqLumSynth –Sapphire
Plasmid#116931PurposeYeast integrative vector for the exression of the 20nm GEM under the HIS3 promoterDepositorInsertlumazine synthase
UseTagsSapphireExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Sec22b
Plasmid#101918PurposeExpresses Sec22b in mammalian cellsDepositorInsertSec22b (Sec22b Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-AMPK alpha1 (1-312)
Plasmid#162131PurposeExpression vector for constitutively active AMPK alpha 1DepositorInsertAMPK alpha1 (1-312) (PRKAA1 Rat)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-ZMYND11
Plasmid#65402PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertZMYND11 (ZMYND11 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Utr230-EGFP-3XNLS
Plasmid#58466PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoterDepositorInsertsUseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB-rtTA
Plasmid#126034PurposePiggyBac cargo vector expressing rtTA. Also confers resistance to G418.DepositorInsertrtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
UsePiggybacTagsExpressionMammalianMutationPromoterhUbiCAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_Luciferase
Plasmid#25894DepositorInsertLuciferase
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Exon1Q23-Myc-B
Plasmid#110488PurposeLentiviral vector to express wild-type N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged wild-type huntingtin exon1 fragment with 23 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationshort 3'UTR mutant huntingtin exon1 fragment…PromoterUbCAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-ZMYND8
Plasmid#65401PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertZMYND8 (ZMYND8 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Grb2 (XSB363)
Plasmid#78505PurposeConstruct for expressing Grb2 in E. ColiDepositorInsertGrb2 (GRB2 Human)
UseTagsGST followed by a prescission protease claevage s…ExpressionBacterialMutationPromoterAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pet16b-Avi-ubiquitin_rbs_BirA
Plasmid#134897PurposeExpresses HIS-Avi-tagged ubiquitin for recombinant protein productionDepositorInsertsUseTags10HIS-AviTag-PreScission(3C)proteaseExpressionBacterialMutationPromoterRBS and T7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor
Plasmid#92142PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.DepositorInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcw107-V5
Plasmid#64649Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertn/a
UseLentiviralTagsV5ExpressionMutationPromoterPGKAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
cpVenus-Gγ2
Plasmid#69626PurposeThe heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).DepositorInsertcp173Venus-Ggamma2 (GNG2 Synthetic)
UseTagsciruclar permutated Venus (cp173V)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
5HT2C-RNA2-Flag
Plasmid#79679Purposeserotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoformDepositorInsertserotonin receptor 2C, RNA2 (HTR2C Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK2 wt
Plasmid#80870Purposemammalian expression of ALK2 WTDepositorInsertALK2 (ACVR1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-GFP-URA3-GFP
Plasmid#72606PurposeTemplate for creating a C-terminal GFP tag AND an internal GFP tag from a single transfection of yeastDepositorInsertsGFP (full)
URA3
GFP (partial)
Optional Linker
UseTagsExpressionMutationIncludes full 819 bp coding sequence of URA3, 435…PromoterAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG3-tdT
Plasmid#62810PurposepPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223_LacZ
Plasmid#25893DepositorInsertLacZ
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-HA-CRBN-pGK-HYG
Plasmid#107378PurposeLentiviral expression of HA-CRBNDepositorInsertcereblon (CRBN Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterUbcPAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-His-tagged-PSPH
Plasmid#134786PurposeStable overexpression lentiviral vector of His tagged PSPHDepositorInsertPhosphoserine phosphatase (PSPH Human)
UseLentiviralTagsHisExpressionMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-neo-PP1-Alpha-H248K
Plasmid#171268PurposeMammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activityDepositorInsert3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)
UseTagsFlagExpressionMammalianMutationPPP1CA-His248Lys (H248K)PromoterCMVAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-TRIM28
Plasmid#45570DepositorInsertTRIM28 (TRIM28 Human)
UseTagsGSTExpressionBacterialMutationPromoterPtacAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits