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pDN623

Plasmid

#175377

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, HIS3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN624

Plasmid

#175378

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, TRP1-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN625

Plasmid

#175379

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, LEU2-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN626

Plasmid

#175380

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, URA3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-Sgd1

Plasmid

#100000

Purpose

Shuttle Sgd1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Sgd1 (SGD1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Bms1

Plasmid

#100001

Purpose

Shuttle Bms1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Bms1 (BMS1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ckb1

Plasmid

#99981

Purpose

Shuttle yeast Ckb1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Ckb1 (CKB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ckb2

Plasmid

#99982

Purpose

Shuttle yeast Ckb2 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Ckb2 (CKB2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Utp11

Plasmid

#99984

Purpose

Shuttle yeast Utp11 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp11 (UTP11 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Pfa1

Plasmid

#99999

Purpose

Shuttle yeast Pfa1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Pfa1 (SQS1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Cka1

Plasmid

#99979

Purpose

Shuttle yeast Cka1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Cka1 (CKA1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pNJB99

Plasmid

#51521

Purpose

Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

Zif268:FokI

Use

Gateway entry vector

Tags

NLS

Expression

Mutation

Promoter

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJYDC1

Plasmid

#162458

Purpose

Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the Nterminus

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

HA tag, HDEL sequence, eUnaG2 fluorescent protein…

Expression

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRH030

Plasmid

#159633

Purpose

CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

Depositor

Robert Hertel

Article

Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

Type

Empty backbone

Use

CRISPR; Shuttle vector

Tags

Expression

Mutation

Promoter

Constitutive tracrRNA

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSMT3-M

Plasmid

#26589

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

n/a

Use

Shuttle vector for mycobacteria

Tags

Expression

Mutation

Promoter

Available since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCB591

Plasmid

#141096

Purpose

E. coli-Lactobacilli shuttle vector for recombineering template cloning

Depositor

Chase Beisel

Article

Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2B

Plasmid

#118228

Purpose

Empty E. coli - B. burgdorferi shuttle vector, blasticidin S resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEN-sacB

Plasmid

#196249

Purpose

Plasmid containing the sacB gene

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

attR2-sacB-attL3

Use

Tags

Expression

Bacterial

Mutation

Promoter

sacB promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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FRP2370

Plasmid

#127576

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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FRP2365

Plasmid

#127575

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge