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Showing: 2241 - 2260 of 6225 results
  1. Nup54 no FG-mEGFP494(2)

    Plasmid
    #164271
    Purpose
    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFP
    Depositor
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    deleted the FG region of Nup54
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. Nup54 no FG-mEGFP494(1)

    Plasmid
    #164270
    Purpose
    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFP
    Depositor
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    deleted the FG region of Nup54
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pYPQ265H

    Plasmid
    #149554
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs
    Depositor
    Insert
    hAID-ziSpyMacCas9(D10A)-UGI
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, R221K, N394K, Protospacer interacting domai…
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pYPQ266B

    Plasmid
    #149555
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs
    Depositor
    Insert
    ziSpyMacCas9(D10A)-PmCDA1-UGI
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, R221K, N394K, Protospacer interacting domai…
    Promoter
    Available since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pYPQ262m3

    Plasmid
    #149556
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs
    Depositor
    Insert
    ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, R221K, N394K, Protospacer interacting domai…
    Promoter
    Available since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pBP-J23108

    Plasmid
    #72964
    Purpose
    Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Insert
    J23108 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. pBP-J23114

    Plasmid
    #72965
    Purpose
    Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Insert
    J23114 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  8. pBP-T7_RBS

    Plasmid
    #72978
    Purpose
    T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)
    Depositor
    Insert
    T7+RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  9. pBP-Strep(II)

    Plasmid
    #72984
    Purpose
    Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)
    Depositor
    Insert
    Strep(II) N-terminal tag
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2241 - 2260 of 6225 results