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pLL7.0: Venus-iLID-Mito (From ActA)

Plasmid

#60413

Purpose

Mammalian Expression of Venus-iLID-Mito

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-Mito

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLL7.0: tgRFPt-SSPB WT

Plasmid

#60415

Purpose

Mammalian Expression of tgRFPt-Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

Y11K and A15E

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLL7.0: tgRFPt-SSPB R73Q

Plasmid

#60416

Purpose

Mammalian Expression of tgRFPt-Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

R73Q, Y11K and A15E

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pACS145

Plasmid

#204600

Purpose

Histone H3.3K36M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.3 K36M (H3-3A Human)

Use

Lentiviral

Tags

human histone H3.3 K36M - V5

Expression

Mammalian

Mutation

histone H3.3 K36M

Promoter

CMV

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pACS142

Plasmid

#204598

Purpose

Histone H3.3K9M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

human H3.3 K9M (H3-3A Human)

Use

Lentiviral

Tags

human histone H3.3 K9M - V5

Expression

Mammalian

Mutation

H3.3 K9M

Promoter

CMV

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgPREX1

Plasmid

#86127

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting PREX1

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

gRNA targeting PREX1 (PREX1 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-PRB

Plasmid

#206165

Purpose

pMVP Gateway destination vector (3rd generation lentiviral vector) containing human full length PRB gene

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

Progesteron receptor B (PGR Human)

Use

Lentiviral; Pmvp gateway destination vector

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 hBAX

Plasmid

#129580

Purpose

CRISPR deletion of human BAX

Depositor

Stephen Tait

Article

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs human BAX (BAX Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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IY601: pMVP/Lenti/Blast-DEST

Plasmid

#121849

Purpose

pMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 mBAX

Plasmid

#129577

Purpose

CRISPR deletion of murine BAX

Depositor

Stephen Tait

Article

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs murine BAX (Bax Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgUBA5

Plasmid

#86132

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting UBA5

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting UBA5 (UBA5 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 15, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgUFM1

Plasmid

#86133

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting UFM1

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting UFM1 (UFM1 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgUFSP2

Plasmid

#86134

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting UFSP2

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting UFSP2 (UFSP2 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCR1063

Plasmid

#111091

Purpose

Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

dCas9-KRAB

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLL7.0: Venus-oLID-Mito (From ActA)

Plasmid

#60414

Purpose

Mammalian Expression of Venus-oLID-Mito

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-oLID-Mito

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLL7.0: Venus-oLID-CAAX (from KRas4B)

Plasmid

#60412

Purpose

Mammalian Expression of Venus-oLID-CAAX

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-oLID-CAAX

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

Plasmid

#219823

Purpose

EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)

Depositor

Philipp Maass

Insert

RfxCas13d

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a core promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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TFAP4_BHLH_5-1

Plasmid

#185054

Purpose

Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

TFAP4_5_1_gRNA (Tcfap4 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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TFAP4_BHLH_3-2

Plasmid

#185055

Purpose

Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_5-1 or TFAP4_BHLH_5-2

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

TFAP4_3_2_gRNA (Tcfap4 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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TFAP4_BHLH_5-2

Plasmid

#185056

Purpose

Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

TFAP4_5_2_gRNA (Tcfap4 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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