Skip to main content
Addgene
Showing: 2241 - 2260 of 7929 results

  1. F11-R pEBio

    Plasmid
    #61486
    Purpose
    Expresses full-length F11R (JAM1) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag and biotinylation sequence
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    F11R (F11R Human)
    Use
    Tags
    enzymatic biotinylation sequence and rat CD4 d3+4
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    61486-plasmid-map-sequence-id-100152

    Enlarge

  3. F2-bio-His

    Plasmid
    #52179
    Purpose
    Expresses full-length Prothrombin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    F2 (F2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52179-plasmid-map-sequence-id-80543

    Enlarge

  4. PTPRJ-bio-His

    Plasmid
    #51816
    Purpose
    Expresses full-length Receptor-type tyrosine-protein phosphatase eta precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PTPRJ (PTPRJ Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51816-plasmid-map-sequence-id-79579

    Enlarge

  5. qTAG-C-miRFP670nano3-Blast-H3C2

    Plasmid
    #207784
    Purpose
    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207784-plasmid-map-sequence-id-416184

    Enlarge

  6. PPIB-bio-His

    Plasmid
    #53413
    Purpose
    Expresses full-length Peptidyl-prolyl cis-trans isomerase B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PPIB (PPIB Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53413-plasmid-map-sequence-id-83196

    Enlarge

  7. p20StAD

    Plasmid
    #190268
    Purpose
    Constitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.
    Depositor
    Assaf Mosquna
    Article
    Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2
    Type
    Empty backbone
    Use
    Synthetic Biology; Yeast genome-integrating vector
    Tags
    GAL4-AD
    Expression
    Mutation
    Promoter
    TDH3
    Available since
    Nov. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    190268-plasmid-map-sequence-id-377252

    Enlarge

  8. pNS19-LV-mutRFP-2A-GFP

    Plasmid
    #113716
    Purpose
    Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)
    Depositor
    Gerald Schwank
    Article
    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1
    Insert
    mutRFP-2A-GFP-IRES-Puro
    Use
    Lentiviral; Reporter system
    Tags
    Expression
    Mammalian
    Mutation
    Y64L (mutRFP)
    Promoter
    Available since
    Aug. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113716-plasmid-map-sequence-id-220030

    Enlarge

  10. ICAM2-bio-His

    Plasmid
    #51758
    Purpose
    Expresses full-length Intercellular adhesion molecule 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ICAM2 (ICAM2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51758-plasmid-map-sequence-id-79347

    Enlarge

  13. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47455
    Purpose
    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    Use
    AAV
    Tags
    2A_GFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47455-plasmid-map-sequence-id-69627

    Enlarge

  14. His-DNPH1

    Plasmid
    #203224
    Purpose
    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    DNPH1 (DNPH1 Human)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203224-plasmid-map-sequence-id-400075

    Enlarge

  16. KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1

    Plasmid
    #121831
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    x-dCas9(3.7)/LSD1 (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121831-plasmid-map-sequence-id-237385

    Enlarge

  18. F2RL2-bio-His

    Plasmid
    #51877
    Purpose
    Expresses full-length Proteinase-activated receptor 3 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    F2RL2 (F2RL2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51877-plasmid-map-sequence-id-79839

    Enlarge

  20. TMED9-bio-His

    Plasmid
    #51855
    Purpose
    Expresses full-length Transmembrane emp24 domain-containing protein 9 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TMED9 (TMED9 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51855-plasmid-map-sequence-id-79742

    Enlarge

Showing: 2241 - 2260 of 7929 results