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Plasmid#219403PurposeFactor Xa-responsive elastin-like polypeptide (ELP)DepositorInsertFXa-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-Thr-cleavableELP
Plasmid#219405PurposeThrombin-responsive elastin-like polypeptide (ELP)DepositorInsertThr-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmN-C47A
Plasmid#221367PurposeExpress C47A ChmN in E. coliDepositorInsertchmN-C47A
UseTagsHis6ExpressionBacterialMutationchanged Cys 47 to AlaPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL4del
Plasmid#216744PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 4 (OL4) by examining the variant lacking OL4 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL5del
Plasmid#216745PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 5 (OL5) by examining the variant lacking OL5 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 5 (OL5) with the sequence of [ATFAA] i…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL1del
Plasmid#216741PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 1 (OL1) with the sequence of [INIGGALQ…PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3L-TwStrep
Plasmid#208649Purposefor E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949DepositorInserthuman RPL3L (ENST00000268661) (RPL3L Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationcontain rs753338989, rs746044959, rs752134949, pr…PromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24
Plasmid#208621Purposefor E. coli expression of Flag tagged human RBM24 (ENST00000379052)DepositorInserthuman RBM24 (ENST00000379052) coding region (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-en1dFnCas9GFP
Plasmid#201950PurposeExpression of en1dFnCas9-GFP in bacterial cellsDepositorInsertExpression of en1dFnCas9-GFP in bacterial cells
UseCRISPRTags6x His, HA, NLS, and mEGFPExpressionBacterialMutationE1369R on FnCas9PromoterT7Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-en15dFnCas9GFP
Plasmid#201951PurposeExpression of en15dFnCas9-GFP in bacterial cellsDepositorInsertExpression of en15dFnCas9-GFP in bacterial cells
UseCRISPRTags6x His, HA, NLS, and mEGFPExpressionBacterialMutationE1603H on FnCas9PromoterT7Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIV p24
Plasmid#212195PurposeBacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminusDepositorInsertChain A, Capsid protein p24, HIV type 1
UseTagsPolyhistidineExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_E192K_D193K
Plasmid#214322PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationE192K_D193KPromoterT7Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-AqpZ-ALFA
Plasmid#212310PurposeExpression of AqpZ with ALFA tagDepositorInsertAquaporin Z
UseTagsALFA tag and Strep-Tag IIExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_I48N
Plasmid#214323PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationI48NPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Caspase-4_sheep_catalytic domain
Plasmid#214331PurposeProtein overexpression in bacteriaDepositorInsertCASP4
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 5W
Plasmid#213623PurposeBacterial expression of SUMO-tagged rat syt1 5WDepositorInsertsyt1 5W (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationM173W, F231W, F234W, C277A, V304W, Y364W, I367CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 s7+
Plasmid#213624PurposeBacterial expression of SUMO-tagged rat syt1 s7+DepositorInsertsyt1 s7+ (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationL171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…PromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-FL-syt1 5W
Plasmid#213629PurposeBacterial expression of SUMO-tagged rat FL-syt1 5WDepositorInsertFL-syt1 5W (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationM173W, F231W, F234W, C277A, V304W, Y364W, I367CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-FL-syt7
Plasmid#213630PurposeBacterial expression of SUMO-tagged mouse FL-syt7DepositorInsertFL-syt7 (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_04
Plasmid#194665PurposeProtein production of the split HaloTag based calcium recorder Caprola_04 in bacteriaDepositorInsertCaprola_04
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only