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Plasmid#192962PurposeHeterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CTDepositorInsertTse5
UseTags9xhis + TEV tagExpressionBacterialMutationPromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_miR-290-295KO-gRNA3
Plasmid#172711PurposeCas9 with 5' gRNA for paired CRISPR KO of miR-290-295 clusterDepositorInsertmiR-290-295
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
OTUB1/pRetroX-IRES-DsRed#10
Plasmid#180279PurposePlasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).DepositorInserthuman Otubain-1 (OTUB1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207in
Plasmid#176230PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207out
Plasmid#176231PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Myosin7B-sgRNA
Plasmid#154861PurposeLentiviral expression of Cas9 and sgRNA targeting Myosin7BDepositorInsertMYO7B sgRNA (MYO7B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAL832
Plasmid#119305Purposeprecusor outer membrane protein A, ompA, truncated after amino acid 176DepositorInsertprecursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
UseTagsnoneExpressionBacterialMutationtruncated after amino acid 176, mutations Threoni…PromoterlacAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 gnu
Plasmid#112999PurposeFor protein expression and purification of full-length Drosophila gnuDepositorInsertfull length gnu (gnu Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PsuACR_353-EYFP
Plasmid#111076PurposeAnion channelrhodopsin from Proteomonas sulcata (CCMP704) for expression in mammalian cellsDepositorInsertAnion channelrhodopsin PsuACR_353
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-JEDI-1P-Kv-WPRE
Plasmid#202612PurposeSoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSynDepositorInsertJEDI-1P-Kv
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSaPIPLC His
Plasmid#173810PurposeExpression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertStaphylococcus aureus plc
UseTags6XHisExpressionBacterialMutationnonePromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET16b-PCNA
Plasmid#134898PurposeBacterial expression and purification of human PCNADepositorInsertPCNA (PCNA Human)
UseTagsuntaggedExpressionBacterialMutationfully synthetic, codon optimized for bacterial ex…PromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE
Plasmid#202608PurposeDouble floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInsertJEDI-1P-Kv
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZsGreen1-cMYC/pLVX-Puromycin
Plasmid#180278PurposePlasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).DepositorInsertZsGreen1-cMYC fusion protein MYC (MYC Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only