Addgene

Showing: 2241 - 2260 of 3250 results

Results per page:

Sort results by:

pEGFP-C1-MKLP1 S708/710A

Plasmid

#70153

Purpose

mammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutations

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

S708A, S710A

Promoter

CMV

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-delta133HtrA2 G399S

Plasmid

#16158

Depositor

L. Miguel Martins

Article

Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

G399S

Promoter

T7

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pX330 LC-FKBP-Cas9

Plasmid

#162724

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T-1 hSlp4-a del.SHD

Plasmid

#40067

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deleted SHD domain (1-143)

Promoter

Tac

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
G protein alpha-o-R179C-EE

Plasmid

#55780

Purpose

This is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

G protein alpha-o with constitutively activating R179C mutation (Gnao1 Rat)

Use

Tags

EE epitope was introduced internally with D167E a…

Expression

Bacterial and Mammalian

Mutation

Arginine 179 was replaced with cysteine

Promoter

CMV

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDSHA-ARL6-Myc

Plasmid

#67359

Purpose

Mammalian expression of hArl6 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-MKLP1 S708A

Plasmid

#70146

Purpose

mammalian expression of MKLP1 with an eGFP fusion and an S708A mutation

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

S708A

Promoter

CMV

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pWzl_neo_DEST_flag_PIK3CA

Plasmid

#45308

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL6-V5-His6

Plasmid

#67422

Purpose

Expression of hArl6 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
HTRA2_PDZ_1

Plasmid

#103913

Purpose

Protein expression and purification of HTRA2 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

HTRA2 (HTRA2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK-FLAG-TRAF4(K190R)

Plasmid

#58317

Purpose

FLAG-tagged mouse TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pGEX4T-1 hSlp4-a V21A

Plasmid

#40064

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Valine 21 to Alanine

Promoter

Tac

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Tubulin-6

Plasmid

#55097

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Use

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-MKLP1 S710A

Plasmid

#70149

Purpose

mammalian expression of MKLP1 with an eGFP fusion and an S710A mutation

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

S710A

Promoter

CMV

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-ARL6-no stop

Plasmid

#67329

Purpose

hArl6 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL6-V5-His6

Plasmid

#67443

Purpose

Mammalian expression of hArl6 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_ARPM1

Plasmid

#45259

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

ARPM1 (ACTRT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-TRAF4(K190R)

Plasmid

#58319

Purpose

EGFP-tagged TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-OposCharlie2

Plasmid

#217169

Purpose

Mammalian expression vector for expression of OposCharlie2 transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

OposCharlie2 transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-TC1_FR4

Plasmid

#217148

Purpose

Mammalian expression vector for expression of TC1_FR4 transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TC1_FR4 transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge