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Showing: 3361 - 3390 of 156570 results

  1. hfCas12Max

    Plasmid
    #195336
    Purpose
    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized hfCas12Max
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R, E336R, D892R
    Promoter
    CBh, CMV, hU6
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    195336-plasmid-map-sequence-id-389250

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  7. pCDB179-TurboPETase

    Plasmid
    #218693
    Purpose
    Encodes codon optimized TurboPETase enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    TurboPETase
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218693-plasmid-map-sequence-id-434224

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  12. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118156-plasmid-map-sequence-id-239644

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  13. TFORF2841

    Plasmid
    #143380
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    IKZF2 (IKZF2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143380-plasmid-map-sequence-id-400282

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  16. KLHL12

    Plasmid
    #38908
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KLHL12 (KLHL12 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pCS2+HyPer7-NLS

    Plasmid
    #136468
    Purpose
    Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
    Depositor
    Vsevolod Belousov
    Article
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Insert
    HyPer7
    Use
    Tags
    Triple nuclear localization signal of SV40 (simia…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SP6
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    136468-plasmid-map-sequence-id-308715

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  25. EGFP-TRAF1

    Plasmid
    #111199
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TRAF1 (TRAF1 Human)
    Use
    Tags
    EGFP-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111199-plasmid-map-sequence-id-213113

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  27. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid
    #174665
    Purpose
    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter
    Depositor
    Eli Bar
    Inserts
    LUC2
    mCherry:T2A:Hygro
    Use
    Lentiviral and Luciferase
    Tags
    mCherry and hygromycin dual reporter gene linked …
    Expression
    Mammalian
    Mutation
    Promoter
    Human cytomegalovirus immediate early enhancer/pr…
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174665-plasmid-map-sequence-id-344886

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  29. TFORF1023

    Plasmid
    #142718
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXI1 (FOXI1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    142718-plasmid-map-sequence-id-462888

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Showing: 3361 - 3390 of 156570 results