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pDF0237 pCMV - dead huDisCas7-11 - NES - huADAR2

Plasmid

#172510

Purpose

Encodes human codon optimized catalytically dead DisCas7-11-NES-huADAR2 fusion protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - dead huDisCas7-11 - NES - huADAR2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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TPC2-G-GECO1.2

Plasmid

#207141

Purpose

Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

TPC2N2 (TPCN2 Human)

Use

Tags

G-GECO1.2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMGF182

Plasmid

#97006

Purpose

Expresses GFP-CHMP7 in mammalian cells under a crippled promoter

Depositor

Wesley Sundquist, Adam Frost

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

CHMP7 (CHMP7 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV delta 5

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-FOXM1-shRNA-81

Plasmid

#89495

Purpose

Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.

Depositor

Adam Karpf

Insert

FOXM1 (FOXM1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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Pmec-18 ced-3 (p15)::nz [TU#806]

Plasmid

#16080

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

ced-3 (p15) :: nz (ced-3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

Plasmid

#101215

Purpose

Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K848A/K1003A/R1060A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K848A, K1003A and R1060A

Promoter

T7

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE2 mCherry-Atl3

Plasmid

#86680

Purpose

Lentivirus to stably express fluorescent protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

ATL3 (ATL3 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-FOXP1

Plasmid

#153145

Purpose

Expression of Flag tagged human FOXP1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXP1 (FOXP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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p256

Plasmid

#186184

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 256

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTK

Plasmid

#165841

Purpose

ganciclovir sensitivity marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

SR39TK

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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