-
Plasmid#112254PurposeExpresses the S*5-M5 apta-FRET RNA origami structure.DepositorInsertS*5-M5 apta-FRET RNA origami with T7 promoter and terminators.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
p8291 MSCV-Neo C-HA 18E7 ΔDLLC
Plasmid#184523PurposeExpression of HPV18 E7 ΔDLLCDepositorInsertHPV18 E7 ΔDLLC
UseRetroviralTagsHAExpressionMutationdeletion of DLLCPromoterMSCV LTRAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8322 pDONR ATG-YAP1 S94A-Stop
Plasmid#184526PurposeSubcloning YAP1 S94ADepositorInsertYAP1 S94A (YAP1 Human)
UseUnspecifiedTagsExpressionMutationS94APromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8328 pLIX YAP1 S94A
Plasmid#184529PurposeExpression of YAP1 S94ADepositorInsertYAP1 S94A (YAP1 Human)
UseLentiviralTagsExpressionMutationS94APromoterTRE promoter, Tet ONAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8321 pDONR ATG-YAP1 S127A-Stop
Plasmid#184525PurposeSubcloning YAP1 S127ADepositorInsertYAP1 S127A (YAP1 Human)
UseUnspecifiedTagsExpressionMutationS127APromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8319 pDONR ATG-YAP1 WT-Stop
Plasmid#184524PurposeSubcloning YAP1 WTDepositorInsertYAP1 WT (YAP1 Human)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pET21b-LaG9(S151C)
Plasmid#172490PurposeEncodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).DepositorInsertLaG9(S151C)
UseTags6xHis, PPX cleavage site, and pelB leader sequenc…ExpressionBacterialMutationS151CPromoterT7Available sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm1_A
Plasmid#145886PurposeInsect Expression of Dm-Lsm1DepositorInsertDm-Lsm1
UseTagsExpressionInsectMutationTwo silent mutation compared to the sequence give…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm4_A
Plasmid#145889PurposeInsect Expression of Dm-Lsm4DepositorInsertDm-Lsm4
UseTagsExpressionInsectMutationone non silent mutation G127R compared to the seq…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm7_A
Plasmid#145891PurposeInsect Expression of DmLSm7DepositorInsertDmLSm7 (LSm7 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-pleCopyCatcher
Plasmid#174061PurposeCopyCatcher insertion donor for the pale locusDepositorInsertPale (Pale Fly, Synthetic)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
UUUUA-pHG165c
Plasmid#170107PurposeConstitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the UreR urea ligand binding domain.DepositorInsertUUUUA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MMMMA-pHG165c
Plasmid#170108PurposeConstitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the MelR L-melibiose ligand binding domain.DepositorInsertMelR
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
XXXXA-pHG165c
Plasmid#170110PurposeConstitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.DepositorInsertXXXXA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
XXXXA_Q182A_R287H-pHG165c
Plasmid#170111PurposeConstitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.DepositorInsertXXXXA_Q182A_R278H
UseTagsExpressionBacterialMutationQ182A, R287HPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
RRRAA-pHG165c
Plasmid#170112PurposeConstitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.DepositorInsertRRRAA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
AraC-pHG165c WT
Plasmid#170114PurposeConstitutively expressed, wild-type E coli AraC transcription activator; activated by L-Arabinose.DepositorInsertAraC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm3_A
Plasmid#145888PurposeInsect Expression of DmLSm3DepositorInsertDmLSm3 (LSm3 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only