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pAAV_EF1a_DIO_HA/FLAG_muMASS_LF

Plasmid

#213394

Purpose

Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS

Plasmid

#213393

Purpose

uMASS_1 opioid sensor. AAV Virus production for Cre dependent expression

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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LLP782_Traptavidin-KRAB-CO

Plasmid

#211775

Purpose

AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-DNMT3A

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_HA_Flag-uMASS_LF

Plasmid

#209761

Purpose

AAV virus production for neuonal expression of uMASS (loss-of-function) under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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LLP786_SpyCatcher-EZH2-FL-CO

Plasmid

#211779

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-KRAB

Use

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP785_Traptavidin-DNMT3A-CO

Plasmid

#211778

Purpose

AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-EZH2

Use

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP781_SnoopCatcher-KRAB-CO

Plasmid

#211774

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-KRAB

Use

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-A-a2-GEMS(PLCG)

Plasmid

#209114

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (PLCG)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a2-GEMS(PLCG)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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(B) NAT marker (R) (SBE173)

Plasmid

#195014

Purpose

nourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+NAT+tNOS

Use

Tags

Expression

Bacterial

Mutation

Promoter

pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(B) G418 marker (R) (SBE 137)

Plasmid

#195011

Purpose

geneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+G418+tNOS

Use

Tags

Expression

Bacterial

Mutation

Promoter

pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-dMASS_LF_WPRE

Plasmid

#209766

Purpose

AAV virus production for neuronal expression of dMASS_LF (loss-of-function) under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_LF

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_HA-Flag-uMASS_1_WPRE

Plasmid

#209760

Purpose

AAV virus production for neuronal expression of uMASS_1 under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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TERTp124_Gluc_CCR5

Plasmid

#192271

Purpose

Donor template for insertion of hTERT promoter (-124 C to T) driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Tags

Expression

Mutation

-124 C to T

Promoter

TERT promoter driving expression of gaussia

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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TERTp146_Gluc_CCR5

Plasmid

#192272

Purpose

Donor template for insertion of hTERT promoter (-146 C to T) driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Tags

Expression

Mutation

-146 C to T

Promoter

TERT promoter driving expression of gaussia

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBeast-pT7-lox

Plasmid

#190059

Purpose

Express the bacterial lactate oxidase enzyme under the control of the strong constitutif phage promoter T7

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

lox

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBeast-pT7-codA

Plasmid

#190058

Purpose

Express the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

codA

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBeast-pYjjZ-RBS- sfGFP

Plasmid

#190054

Purpose

GFP containing reporter plasmid expressing it under the control of the reportedely OxyR/H2O2 inducible pYjjZ promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBeast-pKatG-RBS- sfGFP

Plasmid

#190053

Purpose

GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pKatG promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBeast-pOxyS-RBS- sfGFP

Plasmid

#190052

Purpose

GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pOxyS promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaRII-Cterm-myc

Plasmid

#185505

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Expression

Mutation

RII domain and C-term deleted

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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