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Plasmid#127781PurposeBirA construct in a PCI nls backbone driving mCherry activityDepositorInsertBirA
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-BrainbowTEC
Plasmid#82405PurposeGateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HADepositorInsertstdTomatoMYC fusion
EGFP
E2CrimsonHA
UseTagsHA and MYCExpressionMutationPromoterAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x7
Plasmid#58771PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E-P2A-MCS no-pA
Plasmid#80825PurposeP2A followed by multiple cloning site without pADepositorInsertp3E-P2A-MCS no pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1/Pur
Plasmid#171682Purposeconstitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertOsTIR1-MYC
UseTagsMYCExpressionMammalianMutationPromoterEF-1a promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL1166
Plasmid#129520PurposepDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd GenerationDepositorInsertSpCas9
UsePlasmodium, rodent-infectious speciesTags3XFLAG-NLSExpressionMutationPromoterAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
289 RFP/CFP
Plasmid#83321Purposefluorescent signal joint substrateDepositorInsertRFP +CFP joining substrate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWp9-5
Plasmid#73856PurposepFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1DepositorInsertUBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK571
Plasmid#75379PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseGene synthesisTagsHis6ExpressionBacterialMutationchanges glycine-209 to aspartate and synthetic ge…Promotern/aAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWp9-4
Plasmid#73855PurposepFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1DepositorInsertUBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
P3-Lenti-dCas9-Tet1-GFP
Plasmid#190729PurposeLentiviral construct to express spdCas9-Tet1-GFP.DepositorInsertdCas9-tet1
UseCRISPRTagsSV40 NLS and T2A-EGFPExpressionMammalianMutationChanged Pro434 codon from CCG to CCC and Gly468 f…PromoterUbCAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
p4P1R_EC1.2en-EC1.1p
Plasmid#213912PurposeEntry clone containing Egg cell-specific promoter (EC1.1p) fused to EC1.2 enhancer (EC1.2en). For use in plants and compatible with the MultiSite Gateway systemDepositorInsertEC1.2en-EC1.1p
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover
Plasmid#196829PurposeExpress dpdpCas13b with AAAA mutation and CloverDepositorInsertdpspCas13b(AAAA)
UseTags3XFLAG and NESExpressionMammalianMutationK367A, D369A and K370APromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
aCE89
Plasmid#204494PurposeTREtight promoted WBeta and PhlF promoted PhiC31 without PEST tagsDepositorInsertsφC31 integrase
WBeta recombinase
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterPhIF and TREtightAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
aCE90
Plasmid#204495PurposeTREtight promoted WBeta and PhlF promoted PhiC31 with PEST tagsDepositorInsertsphiC31 recombinase
WBeta recombinase
UseSynthetic BiologyTagsPESTExpressionBacterial and MammalianMutationPromoterPhIF and TREtightAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
aCE106
Plasmid#204496PurposeSmall molecule helper circuitDepositorInsertsrtTA3
PhIF-ABI
VPR-Pyl1
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterhEF1aAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfuNca-MCS
Plasmid#190820PurposeMultiple cloning site between Nitzschia captiva promoter and termination regionsDepositorInsertsMultiple cloning site with SpeI and PstI
Bleomycin resistance gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Zeo
Plasmid#171685Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin)DepositorInsertZeocin
UseTagsExpressionMammalianMutationPromoterRPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCS-TCCC
Plasmid#165774PurposeMultiple cloning site part for enhancer cloning, requires minimal promoter elementDepositorInsertMCS-TCCC
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA
Plasmid#140224PurposeConstitutive PGK driven expression of intein with miniVPR for 3-way split activatorDepositorInsertC-intein with miniVPR
UseTagsmCherryExpressionMammalianMutationPromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only