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Plasmid#166993PurposeTests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E01: attB5r-meGFP-nostop-attB1r
Plasmid#162940PurposeGateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteinsDepositorInsertmonomeric enhanced Green fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E11: attB5r-RU5u-His6-nostop-attB1r
Plasmid#162953PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertHis6 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r
Plasmid#162956PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteinsDepositorInsertHis6-Halotag fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E09: attB5r-RU5u-FLAG-nostop-attB1r
Plasmid#162951PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertFLAG epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E59: attB5r-RU5u-2xHA-nostop-attB1r
Plasmid#162966PurposeGateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteinsDepositorInsertDual HA epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E42: attB5r-RU5u-Strep2-nostop-attB1r
Plasmid#162961PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteinsDepositorInsertStrep2 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E26: attB5r-RU5u-myc-nostop-attB1r
Plasmid#162960PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Myc-tagged fusion proteinsDepositorInsertMyc epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E10: attB5r-RU5u-HA-nostop-attB1r
Plasmid#162952PurposeGateway attB5r/attB1r entry clone for generation of N-terminal HA-tagged fusion proteinsDepositorInsertHA epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E24: attB5r-RU5u-V5-nostop-attB1r
Plasmid#162959PurposeGateway attB5r/attB1r entry clone for generation of N-terminal V5-tagged fusion proteinsDepositorInsertV5 epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E34: attB5r-iRFP670-nostop-attB1r
Plasmid#162947PurposeGateway attB5r/attB1r entry clone for generation of N-terminal iRFP670 fusion proteinsDepositorInsertfar-red iRFP670 fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLY, Linear-3, PolyU(4)
Plasmid#166989PurposeTests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.DepositorInsertTermination module:PolyU(4)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLN, Linear-3, PolyU(4)
Plasmid#166985PurposeTests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.DepositorInsertTermination module:PolyU(4)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0nt
Plasmid#166995PurposeTests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-3_PolyU(1)_Tornado-Corn
Plasmid#159503PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-3_PolyU(1)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-2_PolyU(7)_Tornado-Corn
Plasmid#159493PurposeTests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-2_PolyU(7)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-2_PolyU(4)_Tornado-Corn
Plasmid#159490PurposeTests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-2_PolyU(4)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-2_PolyU(2)_Tornado-Corn
Plasmid#159488PurposeTests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-2_PolyU(2)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-1_PolyU(4)_Tornado-Corn
Plasmid#159482PurposeTests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-1_PolyU(4)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-1_PolyU(5)_Tornado-Corn
Plasmid#159483PurposeTests for the impact of 5 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-1_PolyU(5)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only