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pCS2+-FLAG3-drAgo2DY-EF

Plasmid

#221716

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

D651E and Y680F in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2Y-F

Plasmid

#221715

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Y680F in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2D-E

Plasmid

#221714

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

D651E in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2

Plasmid

#221712

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-FLAG3-TEV

Plasmid

#223875

Purpose

4G-cloning donor for N-terminal His6-FLAG3-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-FLAG3-TEV

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Co-RHD

Plasmid

#180198

Purpose

Expresses FLAG-tagged version of the Co-NF-kB Rel Homology Domain

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-RHD

Use

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-582

Promoter

CMV

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.SLC17A2-flag

Plasmid

#224419

Purpose

Expresses SLC17A2 in mammalian cells

Depositor

Jonathan Long

Insert

Solute carrier family 17 (sodium phosphate), member 2 (SLC17A2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.SLC17A1-flag

Plasmid

#224418

Purpose

Express SLC17A1 in mammalian cells

Depositor

Jonathan Long

Insert

Solute carrier family 17 (sodium phosphate), member 1 (SLC17A1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Human RNF130-3X FLAG

Plasmid

#225331

Purpose

Lentiviral expression of human RNF130 with 3x FLAG tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF130 (RNF130 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Human USP7_H456A-3X FLAG

Plasmid

#225335

Purpose

Lentiviral expression of human mutant USP7 with 3x FLAG tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

USP7 (USP7 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

H456A

Promoter

EF1a

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Human RNF149-3X FLAG

Plasmid

#225332

Purpose

Lentiviral expression of human RNF149 with 3x FLAG tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF149 (RNF149 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pEXP(FLAG/HA-NSP1-WT)

Plasmid

#188781

Purpose

Expresses FLAG/HA-NSP1 in mammalian cells (SARS-CoV-2 NSP1)

Depositor

Markus Hafner

Article

Anastasakis et al PLoS One. 2024 Jan 26;19(1):e0297262. do

Insert

FLAG/HA-NSP1 (SARS-CoV-2)

Use

Frt site to insert into hek293 t-rex genome

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

CMV Dox inducible

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-MAD2-RQ

Plasmid

#221691

Purpose

Mammalian expression of FLAG-tagged human MAD2 (R133E/Q134A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2

Use

Tags

Expression

Mammalian

Mutation

R133E, Q134A

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-DDX53-FLAG

Plasmid

#222241

Purpose

Express FLAG tagged DDX53 in mammalian cells

Depositor

Yuliang Wu

Insert

DDX53 (DDX53 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

T7

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-3*flag-NEXN

Plasmid

#216834

Purpose

expressing Falg tag and the coding sequence of mouse Nexn

Depositor

Yuxuan Guo

Article

Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

Insert

Nexn (mus musculus) (Nexn Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-3xFLAG only

Plasmid

#222243

Purpose

Express 3xFLAG only in mammalian cells as a control

Depositor

Yuliang Wu

Insert

3 x FLAG

Use

Tags

No

Expression

Mammalian

Mutation

Promoter

T7

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CDC50A-c3xFLAG

Plasmid

#215702

Purpose

Produces human CDC50A-c3xFLAG, a chaperone to phosphatidylserine flippase complexes

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CDC50A (TMEM30A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1+FLAG-KrascomQ61R

Plasmid

#206845

Purpose

To express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Codons altered to most optimum based on mouse cod…

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-MAD2

Plasmid

#221690

Purpose

Mammalian expression of FLAG-tagged human MAD2

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-CDC20

Plasmid

#221694

Purpose

Mammalian expression of FLAG-tagged human CDC20

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

CDC20 (CDC20 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge