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Plasmid#146574PurposeMammalian Expression of HsUPF3biso2DepositorInsertHsUPF3biso2 (UPF3B Human)
UseTagsExpressionMammalianMutationone silent mutation T510C compared to the sequenc…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-3-GST-DmGW182_1-593_F
Plasmid#146271PurposeBacterial Expression of DmGW182_1-593DepositorInsertDmGW182_1-593 (gw Fly)
UseTagsExpressionBacterialMutationone non silent mutation T1772A (L591Q) compared t…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-G120E-del133-155-V5-SBP-MBP_V
Plasmid#147767PurposeMammalian Expression of HsSmg5-G120E-del133-155DepositorInsertHsSmg5-G120E-del133-155 (SMG5 Human)
UseTagsExpressionMammalianMutationone silent mutation C969T compared to the sequenc…PromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmEDC3_441-680_D
Plasmid#146127PurposeInsect Expression of DmEDC3_441-680DepositorInsertDmEDC3_441-680 (Edc3 Fly)
UseTagsExpressionInsectMutation3 mutations N162T, K221E and D418G compared to th…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_441-680_D
Plasmid#146130PurposeInsect Expression of DmEDC3_441-680DepositorInsertDmEDC3_441-680 (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-D61A_D
Plasmid#146131PurposeInsect Expression of DmEDC3-D61ADepositorInsertDmEDC3-D61A (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-D61K_D
Plasmid#146132PurposeInsect Expression of DmEDC3-D61KDepositorInsertDmEDC3-D61K (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-N44A_D
Plasmid#146135PurposeInsect Expression of DmEDC3-N44ADepositorInsertDmEDC3-N44A (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD277
Plasmid#125098PurposeExpression of SO_2193(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_2193(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD287
Plasmid#125103PurposeExpression of SO_4637(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4637(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD263
Plasmid#125094PurposeExpression of torR(1-134aa)-ccaR(127-234aa), ompR137 chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertstorR(REC)-ccaR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD266
Plasmid#125096PurposeExpression of torR(1-134aa)-yxdJ(132-229aa), ompR137 chimera under PLtetO-1, output Promoter PyxdJ181DepositorInsertstorR(REC)-yxdJ(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD281
Plasmid#125100PurposeExpression of SO_4428(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4428(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD285
Plasmid#125102PurposeExpression of SO_4623(1-124aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4623(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD283.2
Plasmid#125101PurposeExpression of SO_4487(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4487(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD275
Plasmid#125097PurposeExpression of SO_0622(1-129aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_0622(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PatoL-LOG241
Plasmid#72846PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.DepositorInsertPatoL
UseTagsExpressionBacterialMutationPromoterPatoLAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_SBP-mCherry-Golgin84
Plasmid#65304Purposesynchronize trafficking of Golgin-84 from the ER (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
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