Addgene

Showing: 2221 - 2240 of 3250 results

Results per page:

Sort results by:

pBabe_puro_DEST_Flag_EIF5A2

Plasmid

#45266

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

EIF5A2 (EIF5A2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
Rtn4a-NTD1-135-mNeon

Plasmid

#186609

Purpose

Encodes Reticulon 4 N-terminal domain aa 1 to 135 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 4 isoform A amino acids 1 to 135 (RTN4 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

Plasmid

#184739

Purpose

encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106

Depositor

Randall Platt

Article

Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

Insert

FsRT-Cas1(opt)-Cas2(opt)

Use

Tags

Expression

Bacterial

Mutation

sequence codon optimized for expression in E. coli

Promoter

pTetA

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-delta133HtrA2 A141S

Plasmid

#16157

Depositor

L. Miguel Martins

Article

Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

A141S

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

Plasmid

#130976

Purpose

Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

Depositor

Anne Straube

Article

Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

Insert

Hook3-SNAPf (HOOK3 Human)

Use

Tags

8His-ZZ-LTLT and SNAPf

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FLI1_pet28a

Plasmid

#131641

Purpose

Bacterial expression of ETS gene FLI1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

FLI1 (FLI1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_C_HSP7C

Plasmid

#111667

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

HSP7C (HSPA8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSico_U6-PLOD2 sgRNA4

Plasmid

#136459

Purpose

Expression of gRNA against human PLOD2

Depositor

Antonio Amelio

Article

Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

Insert

gRNA against human PLOD2 (PLOD2 Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330 NC-FKBP-Cas9

Plasmid

#162725

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HtrA2-FLAG S400D

Plasmid

#15940

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S400D

Promoter

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only
MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRES-NeoR

Plasmid

#75136

Purpose

Retroviral expression plasmid encoding inducible shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRES-NeoR

Plasmid

#75135

Purpose

Retroviral expression plasmid encoding inducible shBrd9_1061 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1061

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEE038

Plasmid

#176795

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

407

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEE048

Plasmid

#176805

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

504

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
375 pME-fli1a

Plasmid

#49001

Purpose

full length coding sequence for zebrafish fli1a gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

friend leukemia integration 1a (fli1 Zebrafish)

Use

Gateway middle entry

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMinDis.X513-iNicSnFR3b-(V7)-PM

Plasmid

#125124

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3b-(V7)-PM

Use

Tags

Ig-K-Chain and membrane anchoring protein-PDGFR

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330 L-FKBP-Cas9

Plasmid

#162728

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

Plasmid

#184740

Purpose

encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109

Depositor

Randall Platt

Article

Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

Insert

FsRT-Cas1(opt)-Cas2(opt)

Use

Tags

Expression

Bacterial

Mutation

sequence codon optimized for expression in E. coli

Promoter

pTetA

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mKikGR-Tubulin-6

Plasmid

#57330

Purpose

Localization: Microtubules, Excitation: 507 / 583, Emission: 517 / 593

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Tubulin (TUBA1B Human)

Use

Tags

mKikGR

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3-HtrA2-FLAG S142D

Plasmid

#15939

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S142D

Promoter

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only