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6CDG (GID4)
Plasmid#119961PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 124-289PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETNb-cALFA
Plasmid#159989PurposeFor the protein expression with ALFA tag at C-terminus in E. coliDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Aurein1.2_(+36GFP)-Cre
Plasmid#71748PurposeAurein1.2+36GFP-Cre fusion expression in E. ColiDepositorInsert(GGS)9_Aurein1.2+36GFP-Cre
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
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HDAC6 (3GV4)
Plasmid#25297PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHDAC6_42 (HDAC6 Human)
UseTagsHisExpressionBacterialMutationContains amino acid residues 1109-1215PromoterAvailable sinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
DOT1L
Plasmid#36196PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDOT1L (GeneScript Synthesized) (DOT1L Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationcontains amino acid residues 1-420PromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
PsaA
Plasmid#159997PurposeExpresses Streptococcus pneumoniae PsaA (19-309) in bacterial cellsDepositorInsertPsaA (19-309)
UseTagsHis6-tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
HERC2 (3KCI)
Plasmid#25344PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertherc2.3951.4321 (HERC2 Human)
UseTagsHisExpressionBacterialMutationcontains amino acid residues 3951-4321PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
A0A075HNX4
Plasmid#177695Purposeexpresses a fatty alcohol oxidase in E. coliDepositorInsertLong chain alcohol oxidase
UseTags10xHisExpressionBacterialMutationPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CBX4
Plasmid#25237PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCBX4_02 (CBX4 Human)
UseTags6x His and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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BAT8 (3RJW)
Plasmid#28125PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBAT8 (EHMT2 Human)
UseTagsHis-tag with integrated thrombin siteExpressionBacterialMutationcontains amino acid residues 913-1193.PromoterAvailable sinceFeb. 10, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
Spy0469
Plasmid#159996PurposeExpresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cellsDepositorInsertSpy0469 (24-389)
UseTagsHis6-tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK438
Plasmid#71590PurposeProduces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag (TpCSH6)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pThuS
Plasmid#171674PurposeThuS expression in E. coliDepositorInsertGlycosyltransferase ThuS
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
BRPF1 (3L42)
Plasmid#25339PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBRPF1_P1 (BRPF1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
p3991 pET ubcH9
Plasmid#8651DepositorInsertUbcH9 (UBE2I Human)
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
DLC1A
Plasmid#25333PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDLC1:S1074-L1283 (DLC1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids S1074-L1283PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
Aurein1.2_(pos36GFP)-Cys
Plasmid#71757PurposeAurein1.2_+36GFP-Cys expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-Cys
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEcCas6
Plasmid#170091Purposeexpresses E. coli type I-E Cas6DepositorInsertE. coli type I-E Cas6
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pTH849-gst-optec
Plasmid#89590PurposePlasmid for bacterial expression of His-tagged GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationPromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
SDC229-B03
Plasmid#58763Purposebacterial expression of PDB 4PYZDepositorInsertPDB:4PYZ (USP7 Human)
UseTagsHisExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available sinceAug. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-GST-TNRC6A-(935-984)
Plasmid#42050DepositorInsertTNRC6A (TNRC6A Human)
UseTagsGST and HisExpressionBacterialMutationcontain aa935-984PromoterAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pJK667
Plasmid#71690PurposeProduces E. coli acetate kinase with an N-terminal His6 tag (H6AckA)DepositorInsertacetate kinase (ackA Escherichia coli)
UseTagsHis6ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHA3 (2GSF)
Plasmid#25136PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertepha3.577.947 (EPHA3 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJBL713
Plasmid#140383PurposeExpresses CtcS protein under the control of T7-lacO promoterDepositorInsertT7-lacO-CtcS-T
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only
