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pCS2+-FLAG3-drAgo2Y-F

Plasmid

#221715

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Y680F in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2D-E

Plasmid

#221714

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

D651E in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2_-87prox1a:basEGFP_Acry:GFP

Plasmid

#218213

Purpose

Zebrafish -87prox1a enhancer reporter in the lymphatics at 5 dpf. Acry_GFP as a control in the lens

Depositor

Katarzyna Koltowska

Article

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

-87prox1a

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pME map2k2a_s219D

Plasmid

#201058

Purpose

Gateway middle entry vector expressing constitutively active zebrafish map2k2a

Depositor

Sarah Childs

Article

Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

Insert

MAP2k2a (map2k2a Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

Mutation S219D, constitutively active

Promoter

none

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-mnx1:ReaChR-citrine

Plasmid

#169046

Purpose

Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-Citrine

Use

Tags

Citrine

Expression

Mammalian

Mutation

Promoter

3xMnx1

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-D-kcnj13

Plasmid

#173143

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-kcnj1b

Plasmid

#173126

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj1b (kcnj1b Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnj8

Plasmid

#173134

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj8 (kcnj8, si:dkey-183c2.2 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-Cavin4b-TurboID

Plasmid

#163851

Purpose

In vitro transcription of Cavin4b-TurboID. Parton lab clone JTA

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin4b (cavin4b Zebrafish)

Use

In vitro transcription of rna

Tags

TurboID

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT7gRNA-smyhc1_977

Plasmid

#140867

Purpose

sgRNA synthesis vector for smyhc1_977 (zebrafish slow myosin heavy chain 1).

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

zebrafish smyhc1_977 sgRNA for in vitro transcription (smyhc1 Zebrafish, Synthetic)

Use

In vitro transcription of sgrnas

Tags

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-100-1-dendra2

Plasmid

#105438

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-100-1 Dendra2 (mir100-1 Zebrafish)

Use

Zebrafish

Tags

N/A

Expression

Mutation

Promoter

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-132-2-dendra2

Plasmid

#105439

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-132-2 Dendra2 (mir132-2 Zebrafish)

Use

Zebrafish

Tags

N/A

Expression

Mutation

Promoter

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-429a-dendra2

Plasmid

#105443

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-429a Dendra2 (mir429 Zebrafish)

Use

Zebrafish

Tags

N/A

Expression

Mutation

Promoter

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Dnm2b-DN

Plasmid

#109522

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative Dynamin2b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dnm2b (dnm2b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

DN

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-RhoAa-DN

Plasmid

#109526

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative RhoAa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAa (rhoaa Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

DN

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-RhoAb

Plasmid

#109527

Purpose

Muscle specific zebrafish expression of mKate2 fused to RhoAb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAb (rhoab Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-EHD1b-dEH

Plasmid

#109515

Purpose

Muscle specific zebrafish expression of mKate2 fused to EHD1b-delta-EH

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EHD1b (ehd1b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

dEH

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pME-gata2a

Plasmid

#132977

Purpose

full-length of gata2a without stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

gata2a (gata2a Zebrafish)

Use

Gateway vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDR-nfatc1_sgRNA2

Plasmid

#132978

Purpose

nfatc1 sgRNA targeting to "5'-GTGGGAGCTCCATTGGATCG-3" in pDR274

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

nfatc1 sgRNA2 target sequence

Use

CRISPR; Sgrna generation vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDR-itga9_sgRNA1

Plasmid

#132979

Purpose

itga9 sgRNA targeting to "5'-GAATGACGGAGCTCTCTACG-3" in pDR274

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

itga9 sgRNA1 target sequence

Use

CRISPR; Sgrna generation vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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