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Plasmid#65352PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTDH3p-ycrtE-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_FBXW7:1-707
Plasmid#211104PurposeMammalian expression of full-length human FBXW7. C-terminal HiBiT tag.DepositorInsertFBXW7:M1-K707 (FBXW7 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_FBXW7:1-707
Plasmid#211099PurposeMammalian expression of full-length human FBXW7. N-terminal HiBiT tag.DepositorInsertFBXW7:M1-K707 (FBXW7 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-N_FBXW7:1-707
Plasmid#210946PurposeMammalian expression of full-length human FBXW7. N-terminal NanoLuc luciferase tag.DepositorInsertFBXW7:M1-K707 (FBXW7 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_FBXW7:1-707
Plasmid#210951PurposeMammalian expression of full-length human FBXW7. C-terminal NanoLuc luciferase tag.DepositorInsertFBXW7:M1-K707 (FBXW7 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-N_CCT5:1-541
Plasmid#210978PurposeMammalian expression of full-length human CCT5. N-terminal NanoLuc luciferase tag.DepositorInsertCCT5:M1-E541 (CCT5 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-avi-TEV-LIC_DCAF12:1-453
Plasmid#210940PurposeInsect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.DepositorInsertDCAF12:M1-S453 (DCAF12 Human)
UseTagsExpressionInsectMutationR131Q natural variant (VAR_035322)PromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_EIF3I:1-325
Plasmid#210910PurposeInsect expression of full-length human EIF3I. N-terminal 6X His tag with TEV cleavage.DepositorInsertEIF3I:M1-A325 (EIF3I Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC_DDB1:1-1140_delta:396-689
Plasmid#210897PurposeInsect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.DepositorInsertDDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
UseTagsExpressionInsectMutationloop deletion amino acids I396 to D689PromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_DDB1:1-1140
Plasmid#211147PurposeMammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. N-terminal HiBiT tag.DepositorInsertDDB1:M1-H1140 (DDB1 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationloop deletion I396-D689PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_DDB1:1-1140
Plasmid#211188PurposeMammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. C-terminal HiBiT tag.DepositorInsertDDB1:M1-H1140 (DDB1 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationloop deletion I396-D689PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_CCT5:1-541
Plasmid#210979PurposeMammalian expression of full-length human CCT5. C-terminal NanoLuc luciferase tag.DepositorInsertCCT5:M1-E541 (CCT5 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF.mHES1.CMV.GFP
Plasmid#17622DepositorInsertHES1 (Hes1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSIBR004
Plasmid#177667PurposeSIBR Intron 4 (Int4) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)
Plasmid#71814PurposeExpresses high specificity SpCas9. Px330-like plasmid.DepositorHas ServiceCloning Grade DNAInsertenhanced specificity Cas9 (1.1)
UseCRISPRTags3xFLAGExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA
Plasmid#176270PurposeExpression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cellsDepositorInsertTadA-TadA*(8e)-dCasMINI-V4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
IVT-nCas9(H840A)
Plasmid#207455Purposein vitro transcription of mRNADepositorInsertH840A
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBTK527
Plasmid#110605PurposeBTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK527a
Plasmid#110606PurposeBTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
IVT-R2Tg_opt
Plasmid#223247PurposeIn vitro transcription of the optimized R2Tg proteinDepositorInsertR2Tg protein_opt
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only