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APEX2-csChBP

Plasmid

#108876

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csChBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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APEX2-csGBP

Plasmid

#108874

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csGBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTrcHIS-wt7D12

Plasmid

#125268

Purpose

Expresses wild type 7D12 anti-EGFR nanobodies

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 nanobody

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Trc

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-scFv-VP64_Blast

Plasmid

#192655

Purpose

3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

scFv-VP64

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRVL-4

Plasmid

#104582

Purpose

Contains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human CL kappa

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRVL-1

Plasmid

#104579

Purpose

Contains mouse kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Mouse CL kappa

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHR CD22-Megf10 CAR

Plasmid

#113016

Purpose

for production of lentivirus encoding CD22 CAR-P Megf10

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD22 CAR-P Megf10 intracellular domain

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

Plasmid

#198417

Purpose

Expresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeast

Depositor

Takumi Kamura

Article

Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

Insert

OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1 promoter

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV-VSVgSS-15F11-TM

Plasmid

#207317

Purpose

Functional incorporation of 15F11 scFv (anti-HA) into envelope of virions

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

15F11 ScFv

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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iMbody_pSANG10-3F

Plasmid

#184496

Purpose

Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

Depositor

Marcel Dinger

Article

Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

Insert

iMbody

Use

Tags

3xFLAG, 6xHis, and pelB leader

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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APEX2-ChBP

Plasmid

#108878

Purpose

Expression of APEX2 fused to a nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-ChBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCri_L706-antiSH2-OptoMB

Plasmid

#159935

Purpose

Opto-MB bacterial expression

Depositor

Jose Avalos, Jared Toettcher

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-sNRR-T2A-BFP

Plasmid

#194930

Purpose

LV construct encoding sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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2xMARS-nGFP

Plasmid

#205235

Purpose

Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

Depositor

Jeremy Baskin

Insert

2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)

Use

Tags

HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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CMVTO-Gbp6-TM-Che

Plasmid

#180022

Purpose

Membrane-bound anti-GFP nanobody conjugated to mCherry

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Gpb6 nanobody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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His-tagged FL Rec8

Plasmid

#45243

Purpose

raise antibody against Rec8

Depositor

Michael Lampson

Article

Chiang et al Curr Biol. 2010 Sep 14;20(17):1522-8. do

Insert

Rec8 (Rec8 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-CD4DARPin

Plasmid

#198294

Purpose

Produces PVCs retargeted with anti-CD4 DARPin D27.2

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-CD4DARPin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->Gss52SR4-LaM4-P2A-NLSIFP

Plasmid

#162229

Purpose

Lentiviral vector for the expression of Gss52SR4-LaM4-P2A-NLSIFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG17 GFP nanobody SynNotch tTA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBEVY-4420-mCherry

Plasmid

#218828

Purpose

Yeast surface display of an anti-fluorescene scFv and intracellular expression of mCherry

Depositor

Yongku Cho

Article

Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

Insert

4-4-20 scFv

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBEVY-pT231 scFv-GFP

Plasmid

#218827

Purpose

Yeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFP

Depositor

Yongku Cho

Article

Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

Insert

pT231 tau scFv

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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